Variation in protein intake induces variation in spider silk expression.
Variation in protein intake induces variation in spider silk expression.
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DOI：
10.1371/journal.pone.0031626
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tso IM
中科院分区：
文献类型：
--
作者：
Blamires SJ;Wu CL;Tso IM
合成某些氨基酸需要消耗大量能量。蜘蛛大壶腹 (MA) 丝的蛋白质（蛛丝）MaSp1 和 MaSp2 的氨基酸组成不同。谷氨酰胺和脯氨酸在 MaSp2 中普遍存在，且合成成本昂贵。由于大多数圆网蜘蛛表达高脯氨酸丝，它们可能会优先从食物中获取丝所需的氨基酸，并在饥饿时在其 MA 丝中表达更多 MaSp1。我们喂了三只蜘蛛； Argiope aetherea、Cyrtophora moluccensis 和 Leucauge blanda，高蛋白、低蛋白或无蛋白溶液。 A. aetherea 和 L. blanda MA 丝脯氨酸含量高，而 C. moluccesnsis MA 丝脯氨酸含量低。饲喂 10 天后，我们测定了每个物种的 MA 丝的氨基酸组成和机械性能，并将物种和处理之间的结果与预处理样品进行比较，以解释血统。我们发现A. aetherea和L. blanda丝的脯氨酸和谷氨酰胺受到蛋白质摄入量的影响；在低蛋白质摄入和无蛋白质摄入治疗下显着下降。 C. moluccensis 丝中的谷氨酰胺成分同样受到蛋白质摄入量的影响。然而，MA丝中脯氨酸的组成并没有受到蛋白质摄入量的显着影响。我们的结果表明，蛋白质限制会导致转向谷氨酰胺和/或脯氨酸含量较低的不同丝蛋白。与 MaSp 模型的矛盾在于以下发现：在低/无蛋白质的情况下，C. moluccensis MA 丝没有经历脯氨酸的显着减少，而 A. aetherea 没有经历丝氨酸的显着减少。丝的机械性能不能用 MaSp1 表达变化来解释。 MaSp 表达以外的因素，例如蛛丝蛋白样直系同源物的表达，可能会影响丝的氨基酸组成，而纺丝和腺体过程可能会影响力学。
It is energetically expensive to synthesize certain amino acids. The proteins (spidroins) of spider major ampullate (MA) silk, MaSp1 and MaSp2, differ in amino acid composition. Glutamine and proline are prevalent in MaSp2 and are expensive to synthesize. Since most orb web spiders express high proline silk they might preferentially attain the amino acids needed for silk from food and shift toward expressing more MaSp1 in their MA silk when starved. We fed three spiders; Argiope aetherea, Cyrtophora moluccensis and Leucauge blanda, high protein, low protein or no protein solutions. A. aetherea and L. blanda MA silks are high in proline, while C. moluccesnsis MA silks are low in proline. After 10 days of feeding we determined the amino acid compositions and mechanical properties of each species' MA silk and compared them between species and treatments with pre-treatment samples, accounting for ancestry. We found that the proline and glutamine of A. aetherea and L. blanda silks were affected by protein intake; significantly decreasing under the low and no protein intake treatments. Glutmaine composition in C. moluccensis silk was likewise affected by protein intake. However, the composition of proline in their MA silk was not significantly affected by protein intake. Our results suggest that protein limitation induces a shift toward different silk proteins with lower glutamine and/or proline content. Contradictions to the MaSp model lie in the findings that C. moluccensis MA silks did not experience a significant reduction in proline and A. aetherea did not experience a significant reduction in serine on low/no protein. The mechanical properties of the silks could not be explained by a MaSp1 expressional shift. Factors other than MaSp expression, such as the expression of spidroin-like orthologues, may impact on silk amino acid composition and spinning and glandular processes may impact mechanics.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/s0141-8130(99)00006-9
发表时间：
1999-03-01
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