Antimicrobial Resistance, Virulence Factors, and Genotypes of Enterococcus faecalis and Enterococcus faecium Clinical Isolates in Northern Japan: Identification of optrA in ST480 E. faecalis.
Antimicrobial Resistance, Virulence Factors, and Genotypes of Enterococcus faecalis and Enterococcus faecium Clinical Isolates in Northern Japan: Identification of optrA in ST480 E. faecalis.
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DOI：
10.3390/antibiotics12010108
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发表时间：
2023-01-06
影响因子：
4.8
通讯作者：
Kobayashi, Nobumichi
中科院分区：
文献类型：
--
作者：
Aung, Meiji Soe;Urushibara, Noriko;Kawaguchiya, Mitsuyo;Ohashi, Nobuhide;Hirose, Mina;Kudo, Kenji;Tsukamoto, Naoyuki;Ito, Masahiko;Kobayashi, Nobumichi
关键词：
粪肠球菌（Enterococcus faecalis）和E.屎肠球菌是引起社区和卫生保健相关感染的主要病原体，能够获得对多种抗菌剂的耐药性。本研究旨在了解大肠埃希菌的毒力因子、耐药性及其遗传决定因素和克隆谱系。粪肠球菌E.日本北方屎肠菌临床分离株。共480个（426 E. faecalis和54株E.粪菌）分离物进行分析。三种促进细菌定殖的毒力因子（asa 1、efaA和ace）在E. faecalis（46-59%）比E.肠球菌表面蛋白基因（ESP）在这些种属中的流行率相似。E.粪肠球菌E.屎肠球菌对红霉素、庆大霉素和左氧氟沙星的耐药性及其相关的耐药决定因素存在明显差异。在1株大肠杆菌中检测到恶唑烷酮抗性基因optrA和酚类输出基因fexA。粪肠球菌属于ST 480，并显示位于一个集群类似的分离株在其他亚洲国家的报告。急诊粪肠球菌分离株可分化为12个ST，其中CC 16的ST 179和ST 16是主要的ST谱系。几乎所有的E。屎肠分离株被分配到CC 17中，其由10种不同的序列类型（ST）组成，包括含有多药耐药分离株的显性ST 17和含有透明质酸酶基因（hyl）的分离株的ST 78。本研究揭示了E.粪肠球菌E.在ST 480 E.日本的粪。
Enterococcus faecalis and E. faecium are the major pathogens causing community- and healthcare-associated infections, with an ability to acquire resistance to multiple antimicrobials. The present study was conducted to determine the prevalence of virulence factors, drug resistance and its genetic determinants, and clonal lineages of E. faecalis and E. faecium clinical isolates in northern Japan. A total of 480 (426 E. faecalis and 54 E. faecium) isolates collected over a four-month period were analyzed. Three virulence factors promoting bacterial colonization (asa1, efaA, and ace) were more prevalent among E. faecalis (46–59%) than E. faecium, while a similar prevalence of enterococcal surface protein gene (esp) was found in these species. Between E. faecalis and E. faecium, an evident difference was noted for resistance to erythromycin, gentamicin, and levofloxacin and its responsible resistance determinants. Oxazolidinone resistance gene optrA and phenicol exporter gene fexA were identified in an isolate of E. faecalis belonging to ST480 and revealed to be located on a cluster similar to those of isolates reported in other Asian countries. The E. faecalis isolates analyzed were differentiated into 12 STs, among which ST179 and ST16 of clonal complex (CC) 16 were the major lineage. Nearly all the E. faecium isolates were assigned into CC17, which consisted of 10 different sequence types (STs), including a dominant ST17 containing multidrug resistant isolates and ST78 with isolates harboring the hyaluronidase gene (hyl). The present study revealed the genetic profiles of E. faecalis and E. faecium clinical isolates, with the first identification of optrA in ST480 E. faecalis in Japan.
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DOI：
10.3390/antibiotics11070857
发表时间：
2022-06-26
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.2
作者：
Harada, Sohei;Shibue, Yasushi;Tateda, Kazuhiro
通讯作者：
Tateda, Kazuhiro
影响因子：
5.2
作者：
Deshpande, L. M.;Castanheira, M.;Mendes, R. E.
通讯作者：
Mendes, R. E.
中国杭州无症状健康人粪便中携带 optrA 阳性肠球菌
影响因子：
14.2
作者：
Cai, J.;Schwarz, S.;Wang, Y.
通讯作者：
Wang, Y.
影响因子：
5.2
作者：
Eisenberger, David;Tuschak, Christian;Valenza, Giuseppe
通讯作者：
Valenza, Giuseppe