Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing.
Dissecting the microenvironment around biosynthetic scaffolds in murine skin wound healing.
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DOI：
10.1126/sciadv.abf0787
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发表时间：
2021-05
期刊：
影响因子：
13.6
通讯作者：
Man Y
中科院分区：
文献类型：
--
作者：
Hu C;Chu C;Liu L;Wang C;Jin S;Yang R;Rung S;Li J;Qu Y;Man Y
具有对齐纤维的支架促进小鼠皮肤伤口愈合中的T细胞主导的适应性免疫应答。生物材料的结构特性在指导细胞行为和影响针对材料的免疫反应方面起着至关重要的作用。我们制作了三种类型的表面形貌（随机，对齐，和格子）的电纺膜，将它们引入到小鼠和大鼠的背部皮肤切除伤口，并评估其对伤口愈合和免疫调节特性的影响。借助单细胞RNA测序对微环境中不同免疫细胞的概述揭示了体内不同的细胞异质性。免疫应答的时间进程通过对齐的支架向适应性免疫主导阶段推进。在没有成熟T淋巴细胞的小鼠中，缺乏创伤诱导的毛发新生表明T细胞对毛囊再生的调节作用。支架周围的微环境涉及免疫细胞和皮肤细胞的复杂相互作用。
Scaffolds with aligned fibers promoted a T cell-dominant adaptive immune response in murine skin wound healing. The structural properties of biomaterials play crucial roles in guiding cell behavior and influencing immune responses against the material. We fabricated electrospun membranes with three types of surface topography (random, aligned, and latticed), introduced them to dorsal skin excisional wounds in mice and rats, and evaluated their effects on wound healing and immunomodulatory properties. An overview of different immune cells in the microenvironment with the help of single-cell RNA sequencing revealed diverse cellular heterogeneity in vivo. The time course of immune response was advanced toward an adaptive immunity–dominant stage by the aligned scaffold. In mice without mature T lymphocytes, lack of wound-induced hair neogenesis indicated a regulatory role of T cells on hair follicle regeneration. The microenvironment around scaffolds involved an intricate interplay of immune and cutaneous cells.
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影响因子：
23.9
作者：
Joost, Simon;Annusver, Karl;Kasper, Maria
通讯作者：
Kasper, Maria
用于三维细胞微环境工程的功能和仿生材料
影响因子：
62.1
作者：
Huang G;Li F;Zhao X;Ma Y;Li Y;Lin M;Jin G;Lu TJ;Genin GM;Xu F
通讯作者：
Xu F
影响因子：
14
作者：
Kurpinski, Kyle T.;Stephenson, Jacob T.;Janairo, Randall Raphael R.;Lee, Hanmin;Li, Song
通讯作者：
Li, Song
影响因子：
64.8
作者：
Blees, Andreas;Januliene, Dovile;Tampe, Robert
通讯作者：
Tampe, Robert
影响因子：
16.6
作者：
Guerrero-Juarez, Christian F.;Dedhia, Priya H.;Plikus, Maksim V.
通讯作者：
Plikus, Maksim V.