Acute morphine associated alterations in the subcellular location of the AMPA-GluR1 receptor subunit in dendrites of neurons in the mouse central nucleus of the amygdala: comparisons and contrasts with other glutamate receptor subunits.
Acute morphine associated alterations in the subcellular location of the AMPA-GluR1 receptor subunit in dendrites of neurons in the mouse central nucleus of the amygdala: comparisons and contrasts with other glutamate receptor subunits.
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DOI：
10.1002/syn.21673
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发表时间：
2013-10
期刊：
影响因子：
2.3
通讯作者：
Glass, Michael J.
中科院分区：
文献类型：
--
作者：
Beckerman, Marc A.;Ogorodnik, Evgeny;Glass, Michael J.
在杏仁核内，表达AMPA-GluR 1（GluR 1）亚基的AMPA受体在基础谷氨酸信号传导以及与暴露于滥用药物（如阿片类药物）相关的行为中发挥重要作用。虽然GluR 1的超微结构位置是这种蛋白质的一个重要功能特征，但GluR 1的基底分布以及其对急性吗啡的敏感性从未在小鼠杏仁核中央核（CeA）中被表征。应用免疫电镜技术，观察了小鼠脑前区GluR 1的分布及其与μ阿片受体（μOR）的关系。我们还研究了吗啡对其他谷氨酸受体亚基的影响，包括AMPA-GluR 2（GluR 2）和NMDA-NR 1（NR 1）。在未使用过阿片类药物的动物中，GluR 1和μOR存在于不同的神经元群体中，但主要存在于表达任一抗原的排他性标记的体树突结构中，以及共表达两种蛋白质的体树突结构中。与生理盐水处理的动物相比，给予吗啡的小鼠在树突中GluR 1的亚细胞位置上显示出显著差异，而没有μOR的共表达。虽然GluR 2在非μOR表达树突中也显示出类似的变化，但在GluR 2和μOR共表达谱中观察到了对比效应。这些结果为涉及小鼠CeA中GluR 1或μOR活性调节的基础相互作用提供了超微结构基础。此外，他们指出，亚细胞分布的GluR 1被修改的急性阿片类药物的方式，比较，以及对比，与GluR 2。
Within the amygdala, AMPA receptors expressing the AMPA-GluR1 (GluR1) subunit play an important role in basal glutamate signaling as well as behaviors associated with exposure to drugs of abuse like opiates. Although the ultrastructural location of GluR1 is an important functional feature of this protein, the basal distribution of GluR1, as well as its sensitivity to acute morphine, has never been characterized in the mouse central nucleus of the amygdala (CeA). Electron microscopic immunocytochemistry employing visually distinct gold and peroxidase markers was used to explore the distribution of GluR1 and its relationship with the mu-opioid receptor (μOR) in the mouse CeA under basal conditions and after morphine. We also looked at the effect of morphine on other glutamate receptor subunits, including AMPA-GluR2 (GluR2) and NMDA-NR1 (NR1). In opiate naive animals, GluR1 and μOR were present in diverse populations of neuronal profiles, but mainly in somatodendritic structures that expressed exclusive labeling for either antigen, as well as those co-expressing both proteins. Compared to saline treated animals, mice given morphine showed significant differences in the subcellular location of GluR1 in dendrites without co-expression of μOR. Although GluR2 also showed similar changes in non-μOR expressing dendrites, contrasting effects were seen in GluR2 and μOR co-expressing profiles. These results provide the ultrastructural basis for basal interactions involving the modulation of GluR1 or μOR activity in the mouse CeA. Further, they indicate that the subcellular distribution of GluR1 is modified by acute opiates in a manner that compares, as well as contrasts, with GluR2.
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