Altered behavior and digestive outcomes in adult male rats primed with minimal colon pain as neonates.
Altered behavior and digestive outcomes in adult male rats primed with minimal colon pain as neonates.
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DOI：
10.1186/1744-9081-4-28
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发表时间：
2008-07-10
期刊：
影响因子：
--
通讯作者：
Al-Chaer ED
中科院分区：
文献类型：
--
作者：
Wang J;Gu C;Al-Chaer ED
新生儿结肠刺激（CI;疼痛或炎症）在出生后第22天（PND 22）之前给予2周，在大鼠中引起持久的功能障碍，这可以在初始损伤后6个月观察到。本研究观察了不同新生CI频率和持续时间对成年大鼠生长速率、消化结果、探索活动以及结肠和皮肤敏感性的影响。雄性Sprague-Dawley大鼠在PND 8、10或14开始，以不同的时间间隔和不同的持续时间使用重复的结肠直肠扩张（CRD）给予CI。对照大鼠由研究者处理，不进行任何结肠内插入。在成年大鼠上进行了进一步的实验。使用代谢笼测量消化结果（摄食量和饮水量、粪便和尿液排出量）。探索行为进行了测量，使用数字视频跟踪在一个开放的领域。通过测量对剃毛的腹部或后爪施加的机械和热刺激的反应来评估皮肤敏感性。在轻度异氟烷麻醉下，通过记录外斜肌对CRD的肌电反应来测量内脏敏感性。CI和对照组大鼠之间未观察到显著的体重差异。与对照组相比，新生儿CI大鼠的探索行为减少。不同治疗组之间的消化输出量以及躯体和内脏敏感性发生了变化，早期和更频繁的损伤产生了更高的偏离正常值。这些大鼠的行为和消化症状的多样性与功能性胃肠道疾病患者的症状多样性相似，并且与影响生物体中一个以上系统的全球塑料变化一致。
Neonatal colon irritation (CI; pain or inflammation) given for 2 weeks prior to postnatal day 22 (PND22), causes long-lasting functional disorders in rats that can be seen 6 months after the initial insult. This study looked at the effect of varying the frequency and duration of neonatal CI on the rate of growth, digestive outcomes, exploratory activity, and colon and skin sensitivity in adult rats. Male Sprague-Dawley rats were given CI using repeated colorectal distension (CRD) at different time intervals and for varying durations starting at PND 8, 10 or 14. Control rats were handled by the investigator without any intracolonic insertion. Further experiments were done on adult rats. Digestive outcomes (food and water consumption, fecal and urinary outputs) were measured using metabolic cages. Exploratory behavior was measured using digital video tracking in an open field. Cutaneous sensitivity was assessed by measuring the responses to mechanical and heat stimuli applied to the shaved abdomen or hind paws. Visceral sensitivity was measured by recording electromyographic responses, under light isoflurane anesthesia, from the external oblique muscles in response to CRD. No significant weight differences were observed between CI and control rats. Exploratory behavior was reduced in rats with neonatal CI compared to control. Digestive outputs and somatic and visceral sensitivity changed between different treatment groups with earlier and more frequent insults yielding a higher deviation from normal. The diversity of behavioral and digestive symptoms in these rats parallels the diversity of symptoms in patients with functional gastrointestinal disorders and is consistent with global plastic changes affecting more than one system in the organism.
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影响因子：
3.6
作者：
Gonzalez, MI;Albonetti, E;Wilson, CA
通讯作者：
Wilson, CA
DOI：
10.1097/00005176-199810000-00006
发表时间：
1998-10-01
影响因子：
2.9
作者：
Hamid, SA;Di Lorenzo, C;Hyman, PE
通讯作者：
Hyman, PE
影响因子：
1.7
作者：
Lidow, MS;Song, ZM;Ren, K
通讯作者：
Ren, K
影响因子：
5.1
作者：
Anand, KJS;Runeson, B;Jacobson, B
通讯作者：
Jacobson, B
影响因子：
7.4
作者：
ANDREWS, K;FITZGERALD, M
通讯作者：
FITZGERALD, M