Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae.
Mapping the interaction of Snf1 with TORC1 in Saccharomyces cerevisiae.
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映射SNF1与酿酒酵母中TORC1的相互作用。
DOI：
10.1038/msb.2011.80
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发表时间：
2011-11-08
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
--
作者：
营养感知和代谢途径的协调是所有活细胞的重要功能，但在不同环境条件下的协调细节仍然难以捉摸。因此，我们采用系统生物学方法来研究酿酒酵母中Snf1和雷帕霉素复合体1 （TORC1）靶点之间的相互作用。我们发现，与TORC1相比，Snf1在葡萄糖和氨限制条件下调节的生物过程范围更广。我们还发现Snf1具有上调NADP+依赖性谷氨酸脱氢酶（由GDH3编码）在下调条件下的作用，因此也可能在铵同化和氨基酸生物合成中发挥作用，可以认为这是Snf1和TORC1途径的收敛。除了公认的Snf1在调节脂肪酸代谢中的作用外，我们发现TORC1也可能通过调节过氧化物酶体和β-氧化来调节脂肪酸代谢。最后，我们得出结论，在营养受限的条件下，Snf1和TORC1途径之间的直接相互作用不太可能发生，并提出TORC1以独立于Snf1的方式受到抑制。
Nutrient sensing and coordination of metabolic pathways are crucial functions for all living cells, but details of the coordination under different environmental conditions remain elusive. We therefore undertook a systems biology approach to investigate the interactions between the Snf1 and the target of rapamycin complex 1 (TORC1) in Saccharomyces cerevisiae. We show that Snf1 regulates a much broader range of biological processes compared with TORC1 under both glucose- and ammonium-limited conditions. We also find that Snf1 has a role in upregulating the NADP+-dependent glutamate dehydrogenase (encoded by GDH3) under derepressing condition, and therefore may also have a role in ammonium assimilation and amino-acid biosynthesis, which can be considered as a convergence of Snf1 and TORC1 pathways. In addition to the accepted role of Snf1 in regulating fatty acid (FA) metabolism, we show that TORC1 also regulates FA metabolism, likely through modulating the peroxisome and β-oxidation. Finally, we conclude that direct interactions between Snf1 and TORC1 pathways are unlikely under nutrient-limited conditions and propose that TORC1 is repressed in a manner that is independent of Snf1.
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影响因子：
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作者：
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通讯作者：
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