Green Tea Liquid Consumption Alters the Human Intestinal and Oral Microbiome.
Green Tea Liquid Consumption Alters the Human Intestinal and Oral Microbiome.
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饮用绿茶液体会改变人体肠道和口腔微生物组
DOI：
10.1002/mnfr.201800178
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发表时间：
2018-06
影响因子：
5.2
通讯作者：
Shao Z
中科院分区：
文献类型：
--
作者：
Yuan X;Long Y;Ji Z;Gao J;Fu T;Yan M;Zhang L;Su H;Zhang W;Wen X;Pu Z;Chen H;Wang Y;Gu X;Yan B;Kaliannan K;Shao Z
绿茶多酚(GTP)具有抗结直肠癌活性。肠道微生物区系和口腔来源细菌在肠道的定植与结直肠癌的发生有关。GT调节小鼠肠道微生物区系的组成，与抗癌活性相协调。因此，研究了饮用绿茶液(GTL)对健康志愿者(n=12)肠道和口腔微生物群的影响。对健康志愿者的粪便和唾液样本(干预前、服用GTL 2周(每天400毫升)和洗涤一周后)进行的16S测序和通过重建未观察到的状态(PICRUST)进行的群落系统发育调查显示，微生物多样性和核心微生物区系发生了变化，分类清晰的差异(偏最小二乘判别分析[PLS-DA])。对GTL的反应是不可逆的，增加了FIR：BAC(细菌与类杆菌的比率)，增加了SCFA的产生种类，并减少了粪便中细菌内毒素的合成。GTL改变唾液微生物区系，减少与致癌相关的功能通路的丰度。在包含可能的口腔细菌的粪便和唾液微生物群数据集中发现了类似的细菌网络，GTL降低了梭杆菌的粪便水平。有趣的是，Lachnospiraceae和B/E(双歧杆菌与肠杆菌科的比率标记[CR])都与粪便中口腔样细菌网络的存在呈负相关。这些结果表明，GTL的摄入会导致口腔和肠道微生物群的改变。
GTPs (green tea polyphenols) exert anti‐CRC (colorectal cancer) activity. The intestinal microbiota and intestinal colonization by bacteria of oral origin has been implicated in colorectal carcinogenesis. GT modulates the composition of mouse gut microbiota harmonious with anticancer activity. Therefore, the effect of green tea liquid (GTL) consumption on the gut and oral microbiome is investigated in healthy volunteers (n = 12). 16S sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis of both fecal and saliva samples (collected before intervention, after 2 weeks of GTL (400 mL per day) and after a washout period of one week) in healthy volunteers show changes in microbial diversity and core microbiota and difference in clear classification (partial least squares‐discriminant analysis [PLS‐DA]). An irreversible, increased FIR:BAC (Firmicutes to Bacteroidetes ratio), elevated SCFA producing genera, and reduction of bacterial LPS synthesis in feces are discovered in response to GTL. GTL alters the salivary microbiota and reduces the functional pathways abundance relevance to carcinogenesis. Similar bacterial networks in fecal and salivary microbiota datasets comprising putative oral bacteria are found and GTL reduces the fecal levels of Fusobacterium. Interestingly, both Lachnospiraceae and B/E (Bifidobacterium to Enterobacteriacea ratio—markers of colonization resistance [CR]) are negatively associated with the presence of oral‐like bacterial networks in the feces. These results suggest that GTL consumption causes both oral and gut microbiome alterations.
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DOI：
10.1126/science.aan4526
发表时间：
2017-10-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Suda W;Luo C;Kawaguchi T;Motoo I;Narushima S;Kiguchi Y;Yasuma K;Watanabe E;Tanoue T;Thaiss CA;Sato M;Toyooka K;Said HS;Yamagami H;Rice SA;Gevers D;Johnson RC;Segre JA;Chen K;Kolls JK;Elinav E;Morita H;Xavier RJ;Hattori M;Honda K
通讯作者：
Honda K
影响因子：
4.8
作者：
Carmen Collado, Maria;Engen, Phillip A.;Garaulet, Marta
通讯作者：
Garaulet, Marta
肠道菌群受性别和体重指数的影响。
影响因子：
3.7
作者：
Haro C;Rangel-Zúñiga OA;Alcalá-Díaz JF;Gómez-Delgado F;Pérez-Martínez P;Delgado-Lista J;Quintana-Navarro GM;Landa BB;Navas-Cortés JA;Tena-Sempere M;Clemente JC;López-Miranda J;Pérez-Jiménez F;Camargo A
通讯作者：
Camargo A
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
5
作者：
Henning SM;Yang J;Hsu M;Lee RP;Grojean EM;Ly A;Tseng CH;Heber D;Li Z
通讯作者：
Li Z