Pluripotent differentiation in vitro of murine Es-D3 embryonic stem cells
Pluripotent differentiation in vitro of murine Es-D3 embryonic stem cells
复制标题
小鼠Es-D3胚胎干细胞的体外多能分化
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Denry Sato
中科院分区：
文献类型：
--
作者：
A. Toumadje;K. Kusumoto;A. Parton;P. Mericko;L. Dowell;Guozhong Ma;Luping Chen;D. Barnes;J. Denry Sato
摘要虽然 ES-D3 鼠胚胎干细胞系是最早衍生的细胞系之一，但有关这些细胞的体外分化潜力的信息很少。我们使用免疫细胞化学和流式细胞术方法监测 21 天期间的体外 ES-D3 胚状体分化。在缺乏饲养细胞的情况下，通过白血病抑制因子的撤除诱导胚状体细胞的自发分化。发现多能干细胞标记物 Oct-3/4、SSEA-1 和 EMA-1 持续至少 7 天，而原始内胚层标记物细胞角蛋白内切 A 从第 6 天开始表达水平不断增加。这些抗原在胚状体内的定位表明胚胎外胚层和原始内胚层衍生的组织是分离的。还检测到了 III 类 β-微管蛋白和肌节肌球蛋白的局部表达，表明在体外诱导分化后存在所有三个胚胎胚层的代表。
SummaryAlthough the ES-D3 murine embryonic stem cell line was one of the first derived, little information exists on the in vitro differentiation potential of these cells. We have used immunocytochemical and flow cytometric methods to monitor ES-D3 embryoid body differentiation in vitro during a 21-d period. Spontaneous differentiation of embryoid body cells was induced by leukemia inhibitory factor withdrawal in the absence of feeder cells. The pluripotent stem cell markers Oct-3/4, SSEA-1, and EMA-1 were found to persist for at least 7 d, whereas the primitive endoderm marker cytokeratin endo-A was expressed at increasing levels from day 6. The localization of these antigens within the embryoid bodies suggested that embryonic ectoderm- and primitive endoderm-derived tissues were segregated. Localized expression of class III beta-tubulin and sarcomeric myosin also was detected, indicating that representatives of all three embryonic germ layers were present after induction of differentiation in vitro.
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影响因子：
5.3
作者：
KELLER, G;KENNEDY, M;WILES, MV
通讯作者：
WILES, MV
影响因子：
2.7
作者：
BAIN, G;KITCHENS, D;GOTTLIEB, DI
通讯作者：
GOTTLIEB, DI
DOI：
10.1073/pnas.78.12.7634
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
56.9
作者：
Brüstle, O;Jones, KN;McKay, RDG
通讯作者：
McKay, RDG
Flk-1 表达定义了早期胚胎造血前体细胞群。
DOI：
10.1242/dev.124.10.2039
发表时间：
1997
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kabrun,N;Bühring,HJ;Choi,K;Ullrich,A;Risau,W;Keller,G
通讯作者：
Keller,G