High field magnetic resonance microscopy of the human hippocampus in Alzheimer's disease: quantitative imaging and correlation with iron.
High field magnetic resonance microscopy of the human hippocampus in Alzheimer's disease: quantitative imaging and correlation with iron.
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DOI：
10.1016/j.neuroimage.2011.08.019
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发表时间：
2012-01-16
期刊：
影响因子：
5.7
通讯作者：
Dobson, Jon
中科院分区：
文献类型：
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作者：
Antharam, Vijay;Collingwood, Joanna F.;Bullivant, John-Paul;Davidson, Mark R.;Chandra, Saurav;Mikhaylova, Albina;Finnegan, Mary E.;Batich, Christopher;Forder, John R.;Dobson, Jon
我们报告了五个未固定的死后阿尔茨海默病 (AD) 和三个年龄匹配的对照病例的人类海马体中的 R2 和 R2*。来自匹配的 AD 和对照中的相对半球的福尔马林固定组织被包括在内以进行比较。在 600 MHz (14T) 垂直孔磁体中以 MR 显微镜分辨率进行成像，以获得 R2 和 R2*（62 μm × 62 μm 面内，80 μm 切片厚度）以及 250 μm 各向同性分辨率下的 R1。计算每种情况下各个切片的 R1、R2 和 R2* 图，并用于比较 AD 和对照之间的子字段。 AD 和对照组之间 R2 和 R2* 的幅度变化很小，但与对照组相比，AD 中阿蒙尼角和齿状回的方差显着更高 (p < 0.001)。为了研究组织铁与 MRI 参数之间的关系，在成像平面上对每个组织块进行 30 μm 冷冻切片，并使用同步加速器微焦点 X 射线荧光光谱绘制铁分布图。证明 R2 和 R2* 与铁呈正相关。虽然固定组织的研究更容易进行，但固定可以改变组织中的铁状态，从而使未固定组织的测量变得相关。据我们所知，这些数据代表了未固定组织中海马亚区定量成像的进步，并且这些方法有助于直接分析 MRI 参数与铁之间的关系。与对照相比，AD 的方差显着增加，需要在较低的视野和体内进行研究，以确定该参数是否具有临床相关性。
We report R2 and R2* in human hippocampus from five unfixed post-mortem Alzheimer’s disease (AD) and three age-matched control cases. Formalin-fixed tissues from opposing hemispheres in a matched AD and control were included for comparison. Imaging was performed in a 600 MHz (14T) vertical bore magnet at MR microscopy resolution to obtain R2 and R2* (62 μm × 62 μm in-plane, 80 μm slice thickness), and R1 at 250 μm isotropic resolution. R1, R2 and R2* maps were computed for individual slices in each case, and used to compare subfields between AD and controls. The magnitudes of R2 and R2* changed very little between AD and control, but their variances in the Cornu Ammonis and dentate gyrus were significantly higher in AD compared for controls (p < 0.001). To investigate the relationship between tissue iron and MRI parameters, each tissue block was cryosectioned at 30 μm in the imaging plane, and iron distribution was mapped using synchrotron microfocus X-ray fluorescence spectroscopy. A positive correlation of R2 and R2* with iron was demonstrated. While studies with fixed tissues are more straightforward to conduct, fixation can alter iron status in tissues, making measurement of unfixed tissue relevant. To our knowledge, these data represent an advance in quantitative imaging of hippocampal subfields in unfixed tissue, and the methods facilitate direct analysis of the relationship between MRI parameters and iron. The significantly increased variance in AD compared for controls warrants investigation at lower fields and in-vivo, to determine if this parameter is clinically relevant.
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影响因子：
--
作者：
Brar, Sonia;Henderson, David;Zimmerman, Earl A.
通讯作者：
Zimmerman, Earl A.
影响因子：
3.8
作者：
Dobson, J;Grassi, P
通讯作者：
Grassi, P
影响因子：
4.4
作者：
Haacke, E. Mark;Makki, Malek;Ge, Yulin;Maheshwari, Megha;Sehgal, Vivek;Hu, Jiani;Selvan, Madeswaran;Wu, Zhen;Latif, Zahid;Xuan, Yang;Khan, Omar;Garbern, James;Grossman, Robert I.
通讯作者：
Grossman, Robert I.
DOI：
10.1098/rsbl.2003.0012
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hautot, D;Pankhurst, QA;Dobson, J
通讯作者：
Dobson, J
影响因子：
3.3
作者：
Dawe, Robert J.;Bennett, David A.;Schneider, Julie A.;Vasireddi, Sunil K.;Arfanakis, Konstantinos
通讯作者：
Arfanakis, Konstantinos