Convergent genesis of an adult neural crest-like dermal stem cell from distinct developmental origins.
Convergent genesis of an adult neural crest-like dermal stem cell from distinct developmental origins.
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DOI：
10.1002/stem.525
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发表时间：
2010-11
期刊：
影响因子：
5.2
通讯作者：
Miller, Freda D.
中科院分区：
文献类型：
--
作者：
Jinno, Hiroyuki;Morozova, Olena;Jones, Karen L.;Biernaskie, Jeffrey A.;Paris, Maryline;Hosokawa, Ryoichi;Rudnicki, Michael A.;Chai, Yang;Rossi, Fabio;Marra, Marco A.;Miller, Freda D.
皮肤源性前体细胞 (SKP) 是多能真皮干细胞，位于毛囊微环境中，与胚胎神经嵴前体细胞具有相同的特性。在这里，我们询问 SKP 及其内源性真皮前体是否起源于神经嵴，或者它们是否像真皮本身一样起源于多个发育起源。为此，我们使用了两种不同的小鼠 Cre 系，它们允许我们进行谱系追踪：Wnt1-cre，其目标是来自神经嵴的细胞，而 Myf5-cre，其目标是体节起源的细胞。通过将这些 Cre 系与报告小鼠交叉，我们发现面部的内源性毛囊相关真皮前体源自神经嵴，而背干中的内源性毛囊相关真皮前体源自体节，它们产生的 SKP 也是如此。尽管发育起源不同，但这两个位置的 SKP 在功能上相似，甚至在分化为雪旺细胞（一种仅被认为是从神经嵴产生的细胞类型）的能力方面也是如此。使用微阵列对全局基因表达的分析证实，面部和背部的 SKP 表现出非常高的相似性，并且它们也与来自腹侧真皮的 SKP 非常相似，后者具有侧板起源。然而，这些发育上不同的 SKP 也保留了少数反映其发育起源的基因的差异表达。因此，成人神经嵴样真皮前体可以从非神经嵴起源产生，这一发现对体内的许多神经内分泌细胞具有广泛的影响。
Skin-derived precursors (SKPs) are multipotent dermal stem cells that reside within a hair follicle niche and that share properties with embryonic neural crest precursors. Here, we have asked whether SKPs and their endogenous dermal precursors originate from the neural crest or whether, like the dermis itself, they originate from multiple developmental origins. To do this, we used two different mouse Cre lines that allow us to perform lineage tracing: Wnt1-cre, which targets cells deriving from the neural crest, and Myf5-cre, which targets cells of a somite origin. By crossing these Cre lines to reporter mice, we show that the endogenous follicle-associated dermal precursors in the face derive from the neural crest, and those in the dorsal trunk derive from the somites, as do the SKPs they generate. Despite these different developmental origins, SKPs from these two locations are functionally similar, even with regard to their ability to differentiate into Schwann cells, a cell type only thought to be generated from the neural crest. Analysis of global gene expression using microarrays confirmed that facial and dorsal SKPs exhibit a very high degree of similarity, and that they are also very similar to SKPs derived from ventral dermis, which has a lateral plate origin. However, these developmentally distinct SKPs also retain differential expression of a small number of genes that reflect their developmental origins. Thus, an adult neural crest-like dermal precursor can be generated from a non-neural crest origin, a finding with broad implications for the many neuroendocrine cells in the body.
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影响因子：
64.5
作者：
Adameyko, Igor;Lallemend, Francois;Ernfors, Patrik
通讯作者：
Ernfors, Patrik
影响因子：
11.8
作者：
Sauka-Spengler, Tatjana;Meulemans, Daniel;Bronner-Fraser, Marianne
通讯作者：
Bronner-Fraser, Marianne
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
影响因子：
2.7
作者：
Hosokawa, Ryoichi;Urata, Mark;Chai, Yang
通讯作者：
Chai, Yang
影响因子：
5.2
作者：
Hunt, David P. J.;Morris, Paul N.;Chandran, Siddharthan
通讯作者：
Chandran, Siddharthan