Imaging pancreatic cancer using bioconjugated InP quantum dots.
Imaging pancreatic cancer using bioconjugated InP quantum dots.
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DOI：
10.1021/nn8008933
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发表时间：
2009-03-24
期刊：
影响因子：
17.1
通讯作者：
Prasad, Paras N.
中科院分区：
文献类型：
--
作者：
Yong, Ken-Tye;Ding, Hong;Roy, Indrajit;Law, Wing-Cheung;Bergey, Earl J.;Maitra, Anirban;Prasad, Paras N.
In this paper, we report the successful use of non-cadmium based quantum dots (QDs) as highly efficient and non-toxic optical probes for imaging live pancreatic cancer cells. Indium phosphide (core)-zinc sulphide (shell), or InP/ZnS, QDs with high quality and bright luminescence were prepared by a hot colloidal synthesis method in non-aqueous media. The surfaces of these QDs were then functionalized with mercaptosuccinic acid to make them highly dispersible in aqueous media. Further bioconjugation with pancreatic cancer specific monoclonal antibodies, such as anti-claudin 4 and anti-prostate stem cell antigen (anti-PSCA), to the functionalized InP/ZnS QDs, allowed specific in vitro targeting of pancreatic cancer cell lines (both immortalized and low passage ones). The receptor mediated delivery of the bioconjugates was further confirmed by the observation of poor in vitro targeting in non-pancreatic cancer based cell lines which are negative for the claudin-4-receptor. These observations suggest the immense potential of InP/ZnS QDs as non-cadmium based safe and efficient optical imaging nanoprobes in diagnostic imaging, particularly for early detection of cancer.
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DOI：
10.1073/pnas.152463399
发表时间：
2002-10-01
影响因子：
11.1
作者：
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通讯作者：
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