Expression of IbVPE1 from sweet potato in Arabidopsis affects leaf development, flowering time and chlorophyll catabolism

Expression of IbVPE1 from sweet potato in Arabidopsis affects leaf development, flowering time and chlorophyll catabolism
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拟南芥甘薯中 IbVPE1 的表达影响叶片发育、开花时间和叶绿素分解代谢

DOI:
10.1186/s12870-019-1789-8
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发表时间:
2019-05
期刊:
影响因子:
5.3
通讯作者:
Li Zongyun
Li Zongyun
中科院分区:
生物学2区
文献类型:
--
作者:
Jiang Jiaojiao;Hu Jianzhong;Tan Rujiao;Han Yonghua;Li Zongyun

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自发现以来,液泡处理酶(VPE)一直被认为是细胞程序性死亡(PCD)的启动者和参与者,参与植物发育和对生物或非生物胁迫的响应,部分原因是与细胞凋亡调节因子caspase-1相似。然而,最近的研究表明,VPE在番茄中具有额外的功能,特别是在蔗糖积累和果实成熟方面。在此,我们对甘薯VPE的功能进行了初步研究,初步分析了IbVPE1在发育和衰老过程中的表达模式。随后,我们通过在拟南芥中过表达IbVPE1来鉴定其生理功能,表现为叶片大小和数量减少,开花提前,并阐明了其潜在的分子机制。目前的数据表明,VPE基因家族在拟南芥的发育和衰老以及对开花时间、叶片大小和数量以及衰老表型的调节中具有功能。
Since their discovery, vacuolar processing enzymes (VPEs) have consistently been investigated as programmed cell death (PCD) initiators and participants in plant development and responses to biotic or abiotic stresses, in part due to similarities with the apoptosis regulator caspase-1. However, recent studies show additional functions of VPE in tomatoes, specifically in sucrose accumulation and fruit ripening. Herein, we evaluated the functions of VPE from sweetpotato, initially in expression pattern analyses of IbVPE1 during development and senescence. Subsequently, we identified physiological functions by overexpressing IbVPE1 in Arabidopsis thaliana, and showed reduced leaf sizes and numbers and early flowering, and elucidated the underlying molecular mechanisms. The present data demonstrate functions of the VPE gene family in development and senescence and in regulation of flowering times, leaf sizes and numbers, and senescence phenotypes in Arabidopsis thaliana.
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