Toward the construction of integrated physical and genetic maps of the mouse genome using interspersed repetitive sequence PCR (IRS-PCR) genomics.

Toward the construction of integrated physical and genetic maps of the mouse genome using interspersed repetitive sequence PCR (IRS-PCR) genomics.
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使用散布重复序列 PCR (IRS-PCR) 基因组学构建小鼠基因组的综合物理和遗传图谱。

DOI:
10.1101/gr.6.4.290
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发表时间:
1996
期刊:
影响因子:
7
通讯作者:
Housman,D
Housman,D
中科院分区:
生物学1区
文献类型:
--
作者:
Hunter,KW;Riba,L;Schalkwyk,L;Clark,M;Resenchuk,S;Beeghly,A;Su,J;Tinkov,F;Lee,P;Ramu,E;Lehrach,H;Housman,D

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利用IRS-PCR YAC步移和IRS-PCR基因分型技术,构建了小鼠基因组的框架整合物理和遗传图谱。该图谱由821个重叠群组成,含有来自三个不同YAC文库的7746个YAC克隆。380个重叠群已经被锚定在遗传图谱上。据估计,小鼠基因组的物理长度约为16%。
Using two recently developed techniques, IRS-PCR YAC walking and IRS-PCR genotyping, a framework-integrated physical and genetic map of the mouse genome was constructed. The map consists of 821 contigs, containing 7746 YAC clones originating from three different YAC libraries. Three hundred eighty of the contigs have been anchored to the genetic map. Approximately 16% of the physical length of the mouse genome is estimated to be represented.
第一代人类基因组物理图谱
DOI: --
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影响因子: 64.8
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