An improved fruit transcriptome and the identification of the candidate genes involved in fruit abscission induced by carbohydrate stress in litchi.
An improved fruit transcriptome and the identification of the candidate genes involved in fruit abscission induced by carbohydrate stress in litchi.
复制标题
改进的果实转录组和鉴定参与荔枝碳水化合物胁迫诱导果实脱落的候选基因。
DOI：
10.3389/fpls.2015.00439
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发表时间：
2015
影响因子：
5.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li C;Wang Y;Huang X;Li J;Wang H;Li J
荔枝（litchi chinensis Sonn.）是热带和亚热带地区重要的水果作物，幼果大量脱落是荔枝产量低且不稳定的主要原因。然而，对水果糖的分子机制还没有完全的研究。本研究旨在鉴定荔枝果实脱落的相关分子成分，目前还没有相关的参考基因组。通过使用优化的组装软件Trinity，首次实现了改进的从头组装转录组。以改良的转录组组装为参照，采用数字转录丰度（DTA）分析方法筛选和鉴定了环剥加落叶（GPD）诱导果实脱落的候选基因，GPD处理可显著降低果实可溶性糖含量，导致碳水化合物胁迫。结果表明，GPD处理后果实脱落率增加与果实乙烯产量增加和葡萄糖水平降低有关。共有2771个差异表达基因被鉴定为gpd响应基因，其中857个通过GO和KEGG富集分析确定为参与果实脱落过程的候选基因。这些基因参与多种代谢过程和途径，包括碳水化合物代谢、植物激素合成、信号转导、转录因子活性和细胞壁修饰，在早期（处理后2天内）迅速诱导。利用qRT-PCR技术对15个候选基因在脱落区、果皮和种子中的表达模式进行了分析，验证了DTA数据的准确性。还分析了不同功能类别的更详细信息。本研究在全转录组水平上分析了碳水化合物胁迫诱导荔枝果实脱落的相关基因表达，为进一步了解荔枝幼果脱落的调控机制提供了依据。
Massive young fruit abscission usually causes low and unstable yield in litchi (Litchi chinensis Sonn.), an important fruit crop cultivated in tropical and subtropical areas. However, the molecular mechanism of fruit drop has not been fully characterized. This study aimed at identification of molecular components involved in fruitlet abscission in litchi, for which reference genome is not available at present. An improved de novo transcriptome assembly was firstly achieved by using an optimized assembly software, Trinity. Using improved transcriptome assembly as reference, digital transcript abundance (DTA) profiling was performed to screen and identify candidate genes involved in fruit abscission induced by girdling plus defoliation (GPD), a treatment significantly decreased the soluble sugar contents causing carbohydrate stress to fruit. Our results showed that the increasing fruit abscission rate after GPD treatment was associated with higher ethylene production and lower glucose levels in fruit. A total of 2,771 differentially expressed genes were identified as GPD-responsive genes, 857 of which were defined by GO and KEGG enrichment analyses as the candidate genes involved in fruit abscission process. These genes were involved in diverse metabolic processes and pathways, including carbohydrate metabolism, plant hormone synthesis, and signaling, transcription factor activity and cell wall modification that were rapidly induced in the early stages (within 2 days after treatment). qRT-PCR was used to explore the expression pattern of 15 selected candidate genes in the abscission zone, pericarp, and seed, which confirmed the accuracy of our DTA data. More detailed information for different functional categories was also analyzed. This study profiled the gene expression related to fruit abscission induced by carbohydrate stress at whole transcriptome level and thus provided a better understanding of the regulatory mechanism of young fruit abscission in litchi.
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影响因子：
5.3
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
7.4
作者：
Burr, Christian A.;Leslie, Michelle E.;Liljegren, Sarah J.
通讯作者：
Liljegren, Sarah J.
碳水化合物胁迫对荔枝果实脱落及生长素信号转导通路相关基因表达的影响
影响因子：
5.6
作者：
Kuang JF;Wu JY;Zhong HY;Li CQ;Chen JY;Lu WJ;Li JG
通讯作者：
Li JG
影响因子：
48
作者：
Mortazavi, Ali;Williams, Brian A.;Wold, Barbara
通讯作者：
Wold, Barbara
影响因子：
3.7
作者：
Corbacho J;Romojaro F;Pech JC;Latché A;Gomez-Jimenez MC
通讯作者：
Gomez-Jimenez MC