Randomised clinical study: inulin short-chain fatty acid esters for targeted delivery of short-chain fatty acids to the human colon.
Randomised clinical study: inulin short-chain fatty acid esters for targeted delivery of short-chain fatty acids to the human colon.
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DOI：
10.1111/apt.13749
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发表时间：
2016-10
影响因子：
7.6
通讯作者：
Morrison DJ
中科院分区：
文献类型：
--
作者：
Polyviou T;MacDougall K;Chambers ES;Viardot A;Psichas A;Jawaid S;Harris HC;Edwards CA;Simpson L;Murphy KG;Zac-Varghese SE;Blundell JE;Dhillo WS;Bloom SR;Frost GS;Preston T;Tedford MC;Morrison DJ
短链脂肪酸（SCFA）通过肠道菌群发酵不可消化的碳水化合物产生，具有积极的代谢作用。然而，对照良好的人体试验是有限的。开发一种将短链脂肪酸直接输送到结肠的方法，并优化人类结肠丙酸输送，以确定其在食欲调节和食物摄入中的作用。开发并测试了菊粉SCFA酯作为特定部位的SCFA到近端结肠的递送载体。通过体外分批粪便发酵，对含有0-61 wt% （IPE‐0-IPE‐61）丙酸菊粉酯进行了评估。在一项随机、对照、交叉研究中，以菊粉为对照，在IPE - 27或IPE - 54（所有处理均为10克/天）治疗7天后比较任意食物摄入量（kcal）。丙酸释放用13C‐标记的IPE变体测定。在体外实验中，与菊粉相比，IPE‐27-IPE‐54 wt%丙酸盐使丙酸产量增加了7倍（P < 0.05）。在体内，IPE‐27比IPE‐54导致呼吸co2中更高的13C恢复（64.9% vs. 24.9%, P = 0.001）。与菊粉（439.5 kcal vs. 703.9 kcal, P = 0.025）和IPE‐54 （439.5 kcal vs. 659.3 kcal, P = 0.025）相比，IPE‐27也导致了随意试餐期间能量摄入的减少，而IPE‐54与菊粉对照组没有显著差异。IPE‐27显著减少食物摄入，提示结肠丙酸盐在食欲调节中起作用。菊粉短链脂肪酸酯为探索人类饮食-肠道微生物-宿主代谢轴提供了一种新的工具。
Short‐chain fatty acids (SCFA) produced through fermentation of nondigestible carbohydrates by the gut microbiota are associated with positive metabolic effects. However, well‐controlled trials are limited in humans. To develop a methodology to deliver SCFA directly to the colon, and to optimise colonic propionate delivery in humans, to determine its role in appetite regulation and food intake. Inulin SCFA esters were developed and tested as site‐specific delivery vehicles for SCFA to the proximal colon. Inulin propionate esters containing 0–61 wt% (IPE‐0–IPE‐61) propionate were assessed in vitro using batch faecal fermentations. In a randomised, controlled, crossover study, with inulin as control, ad libitum food intake (kcal) was compared after 7 days on IPE‐27 or IPE‐54 (10 g/day all treatments). Propionate release was determined using 13C‐labelled IPE variants. In vitro, IPE‐27–IPE‐54 wt% propionate resulted in a sevenfold increase in propionate production compared with inulin (P < 0.05). In vivo, IPE‐27 led to greater 13C recovery in breath CO 2 than IPE‐54 (64.9 vs. 24.9%, P = 0.001). IPE‐27 also led to a reduction in energy intake during the ad libitum test meal compared with both inulin (439.5 vs. 703.9 kcal, P = 0.025) and IPE‐54 (439.5 vs. 659.3 kcal, P = 0.025), whereas IPE‐54 was not significantly different from inulin control. IPE‐27 significantly reduced food intake suggesting colonic propionate plays a role in appetite regulation. Inulin short‐chain fatty acid esters provide a novel tool for probing the diet–gut microbiome–host metabolism axis in humans.
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影响因子：
6.9
作者：
Daud, Norlida M.;Ismail, Nurhafzan A.;Frost, Gary S.
通讯作者：
Frost, Gary S.
肠道微生物群形成短链脂肪酸及其对人代谢的影响。
影响因子：
12.2
作者：
Morrison DJ;Preston T
通讯作者：
Preston T
影响因子：
3.7
作者：
Maurer, Jacoba M.;Schellekens, Reinout C. A.;Kosterink, Jos G. W.
通讯作者：
Kosterink, Jos G. W.
影响因子：
4.8
作者：
Brown, AJ;Goldsworthy, SM;Dowell, SJ
通讯作者：
Dowell, SJ
DOI：
10.1152/ajpgi.1998.275.6.g1415
发表时间：
1998-12-01
影响因子：
4.5
作者：
Cherbut, C;Ferrier, L;Galimiche, JP
通讯作者：
Galimiche, JP