Shared and genetically distinct Zea mays transcriptome responses to ongoing and past low temperature exposure.
Shared and genetically distinct Zea mays transcriptome responses to ongoing and past low temperature exposure.
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DOI：
10.1186/s12864-018-5134-7
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发表时间：
2018-10-20
期刊：
影响因子：
4.4
通讯作者：
Lukens L
中科院分区：
文献类型：
--
作者：
Avila LM;Obeidat W;Earl H;Niu X;Hargreaves W;Lukens L
低温及其缓解会影响许多植物性状，包括蛋白质编码基因转录本的丰度。响应低温而发生的转录水平变化及其缓解在不同基因型之间是共有的或不同的。在这项研究中，我们确定了对寒冷及其缓解有一致反应的单个转录本和功能相关转录本组。不同基因型对温度变化反应不同的基因可能具有有限的功能重要性。我们研究这些基因是否具有相同的功能，以及它们的基因型特异性基因表达水平是否随温度的变化而变化。我们估计了两个不相关的玉米自交系在低温暴露期间和之后的超过 22,000 个基因的转录本丰度。基因型和温度决定了许多基因的丰度。过去的寒冷暴露影响的基因要少得多。寒冷中上调的基因编码许多细胞分裂素葡萄糖苷生物合成酶、转录因子、信号分子和参与多种环境反应的蛋白质。冷暴露后，蛋白酶抑制剂和角质蜡质基因重新上调，环境响应基因继续上调。因寒冷而下调的基因包括许多与光合作用、翻译和 DNA 复制相关的基因。冷暴露后，DNA复制和翻译基因仍然优先下调。木质素和木栓质生物合成新近下调。 DNA 复制、活性氧反应和花青素生物合成基因具有强烈的基因型特异性温度反应。基因型转录本丰度的排名通常会随着温度的变化而变化。我们报告了对寒冷和寒冷缓解的大型核心转录组反应。在寒冷条件下，许多核心基因组会上调或下调，以控制植物生长和光合作用并限制细胞损伤。在恢复过程中，核心反应在一定程度上是为未来的压力做好准备。在应对寒冷或其缓解的过程中，功能相关的基因在单一基因型中持续大幅上调，这表明正向选择驱动了玉米中基因型特异性的温度反应。本文的在线版本 (10.1186/s12864-018-5134-7) 包含补充材料，可供授权用户使用。
Cold temperatures and their alleviation affect many plant traits including the abundance of protein coding gene transcripts. Transcript level changes that occur in response to cold temperatures and their alleviation are shared or vary across genotypes. In this study we identify individual transcripts and groups of functionally related transcripts that consistently respond to cold and its alleviation. Genes that respond differently to temperature changes across genotypes may have limited functional importance. We investigate if these genes share functions, and if their genotype-specific gene expression levels change in magnitude or rank across temperatures. We estimate transcript abundances from over 22,000 genes in two unrelated Zea mays inbred lines during and after cold temperature exposure. Genotype and temperature contribute to many genes’ abundances. Past cold exposure affects many fewer genes. Genes up-regulated in cold encode many cytokinin glucoside biosynthesis enzymes, transcription factors, signalling molecules, and proteins involved in diverse environmental responses. After cold exposure, protease inhibitors and cuticular wax genes are newly up-regulated, and environmentally responsive genes continue to be up-regulated. Genes down-regulated in response to cold include many photosynthesis, translation, and DNA replication associated genes. After cold exposure, DNA replication and translation genes are still preferentially downregulated. Lignin and suberin biosynthesis are newly down-regulated. DNA replication, reactive oxygen species response, and anthocyanin biosynthesis genes have strong, genotype-specific temperature responses. The ranks of genotypes’ transcript abundances often change across temperatures. We report a large, core transcriptome response to cold and the alleviation of cold. In cold, many of the core suite of genes are up or downregulated to control plant growth and photosynthesis and limit cellular damage. In recovery, core responses are in part to prepare for future stress. Functionally related genes are consistently and greatly up-regulated in a single genotype in response to cold or its alleviation, suggesting positive selection has driven genotype-specific temperature responses in maize. The online version of this article (10.1186/s12864-018-5134-7) contains supplementary material, which is available to authorized users.
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影响因子：
7.2
作者：
Bonaventure, G;Beisson, F;Pollard, M
通讯作者：
Pollard, M
影响因子：
2.9
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