Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition
Effects of breed, postnatal development, and nutrition on mRNA expression of the FTO gene in porcine muscle and its relationship with intramuscular fat deposition
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品种、产后发育及营养对猪肌肉FTO基因mRNA表达的影响及其与肌内脂肪沉积的关系
DOI：
10.17221/6905-cjas
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发表时间：
2018-02
影响因子：
1.2
通讯作者：
Xu, Z. W.
中科院分区：
文献类型：
--
作者：
Tao, X.;Men, X. M.;Deng, B.;Xu, Z. W.
研究了品种、发育和营养对猪肌肉脂肪量和肥胖相关基因（FTO）mRNA表达的影响及其与肌内脂肪（IMF）含量的关系。背最长肌估计。选用金华、中白、约克郡、杜洛克、杜×中白（DZ）、杜×约克郡×长白猪（DYL）5个杂交猪品种，研究品种效应。选择体重为2.5、10、20、40、60和100 kg的猪，研究不同发育阶段的影响。试验选用玉米-大豆（CS）、添加1.2%共轭亚油酸（CLA）或0.05%一水肌酸（CMH）的CS和大麦-大豆（BS）四种日粮，研究其营养效果。所有80只动物都被屠宰了，m.l.d.收集样品以检测FTO mRNA表达和IMF含量。结果表明，品种显著影响FTO mRNA表达和IMF含量。FTO mRNA在各试验猪中的表达量顺序为：中白猪和约克郡>杜洛克猪和DZ猪>金华猪和DYL猪。IMF含量按品种排序为杜洛克> DZ > DYL >金华>中白>约克郡。FTO mRNA表达量和IMF含量均随猪龄增加而增加，其中100 kg组与其他体重组差异最大。在本研究中，四种日粮对FTO mRNA表达和IMF含量均无显著影响（P > 0.05）。结果表明，FTO mRNA表达量随体重的增加而增加，且受品种的影响显著。结果表明，FTO mRNA表达与IMF含量在品种和发育年龄间存在不一致的相关性。
The effects of breed, development, and nutrition on mRNA expression of the fat mass and obesity- associated gene ( FTO) and its relationship with intramuscular fat (IMF) content in porcine muscle ( m. longissimus dorsi; m.l.d.) were estimated . Purebred Jinhua, Zhongbai, Yorkshire, Duroc, Duroc × Zhongbai (DZ), and Duroc × Yorkshire × Landrace (DYL) pigs were used to investigate the effect of breed. Pigs weighing 2.5, 10, 20, 40, 60, and 100 kg were selected to study the effects of different stages of development. To study the effect of nutrition, four diets were selected: corn-soybean (CS), CS with 1.2% conjugated linoleic acid (CLA) or 0.05% creatine monohydrate (CMH), and barley-soybean (BS). All eighty animals were slaughtered, and m.l.d. samples were collected to examine FTO mRNA expression and IMF content. Results showed that breed significantly affected FTO mRNA expression and IMF content. FTO mRNA expression in the studied pigs was in the order: Zhongbai and Yorkshire > Duroc and DZ > Jinhua and DYL. The IMF content ordered by breed was Duroc > DZ > DYL > Jinhua > Zhongbai > Yorkshire. Both FTO mRNA expression and IMF content increased with age of the pigs, with the greatest difference seen between 100 kg pigs and all other weights. In the study, none of the four diets had a significant effect ( P > 0.05) on FTO mRNA expression or IMF content. The study demonstrated that FTO mRNA expression increased with increasing body weight and was significantly affected by the breed of pigs. The results showed that FTO mRNA expression had an inconsistent correlation with IMF content between breeds and developmental ages.
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影响因子：
3.7
作者：
McTaggart JS;Lee S;Iberl M;Church C;Cox RD;Ashcroft FM
通讯作者：
Ashcroft FM
影响因子：
4.6
作者：
F. Hoeven;T. Schimmang;A. Volkmann;M. Mattei;B. Kyewski;U. Rüther
通讯作者：
F. Hoeven;T. Schimmang;A. Volkmann;M. Mattei;B. Kyewski;U. Rüther
影响因子：
2.1
作者：
Tyra, M.;Ropka-Molik, K.;Oczkowicz, M.
通讯作者：
Oczkowicz, M.
影响因子：
7.1
作者：
Cordero, G.;Isabel, B.;Lopez-Bote, C. J.
通讯作者：
Lopez-Bote, C. J.
影响因子：
3.3
作者：
F. Gerbens;F. Verburg;H. V. van Moerkerk;B. Engel;W. Buist;J H Veerkamp;M F te Pas
通讯作者：
F. Gerbens;F. Verburg;H. V. van Moerkerk;B. Engel;W. Buist;J H Veerkamp;M F te Pas