Rapid determination of baicalin and total baicalein content in Scutellariae radix by ATR-IR and NIR spectroscopy.
Rapid determination of baicalin and total baicalein content in Scutellariae radix by ATR-IR and NIR spectroscopy.
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ATR-IR和NIR光谱法快速测定黄芩中黄芩苷和总黄芩素含量
DOI：
10.1016/j.talanta.2013.05.046
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发表时间：
2013-09-30
期刊：
影响因子：
6.1
通讯作者：
Huck CW
中科院分区：
文献类型：
--
作者：
Navarro Escamilla M;Rodenas Sanz F;Li H;Schönbichler SA;Yang B;Bonn GK;Huck CW
In this study methods for the quantification of baicalin and total baicalein in Scutellariae radix with near infrared (NIR) spectroscopy and attenuated-total-reflectance mid-infrared (ATR-IR) spectroscopy in hyphenation with multivariate analysis were developed and compared. The reference analysis was performed by high performance liquid chromatography coupled to diode array detection (HPLC-DAD). Different pretreatments like standard normal variate (SNV), multiplicative scatter correction (MSC), first and second derivative Savitzky-Golay were applied on the spectra to optimize the calibrations. A principal component analysis was performed with both spectroscopic methods to distinguish wild and cultivated samples. Quality parameters obtained for test-set calibration models of ATR-IR spectroscopy (baicalin: standard error of prediction (SEP)=1.31, ratio performance to deviation (RPD)=2.91 and R2=0.88; total baicalein: SEP=1.02, RPD=3.24 and R2=0.89) and NIR spectroscopy (baicalin: SEP=1.50, RPD=2.54 and R2=0.88; total baicalein: SEP=1.19, RPD=2.76 and R2=0.84) demonstrate that both spectroscopic techniques in combination with multivariate analysis are successful tools for the quantification of baicalin and total baicalein in Scutellariae radix, but it was found that ATR-IR spectroscopy provides higher accuracy in the given application. Furthermore it was proved that wild and cultivated samples can be distinguished by ATR-IR. ATR-IR and NIR spectroscopic methods were developed and compared. Baicalin and total baicalein were determined in Scutellaria baicalensis. ATR-IR and NIR spectroscopy are suitable to determine the baicalin and total baicalein content. ATR-IR reveals better quality parameters than NIR spectroscopy. Cultivated and wild-type samples could be distinguished by PCA analysis of ATR-IR spectra.
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影响因子：
9.7
作者：
MOTOO, Y;SAWABU, N
通讯作者：
SAWABU, N
影响因子：
7.4
作者：
SAVITZKY, A;GOLAY, MJE
通讯作者：
GOLAY, MJE
影响因子：
6.2
作者：
LIU, YM;SHEU, SJ
通讯作者：
SHEU, SJ
影响因子：
3.5
作者：
Rodriguez-Saona, LE;Koca, N;Alvarez, VB
通讯作者：
Alvarez, VB
影响因子：
2.1
作者：
Ikemoto, S;Sugimura, K;Kishimoto, T
通讯作者：
Kishimoto, T