Quantitative trait loci analysis of fiber quality traits using a random-mated recombinant inbred population in Upland cotton (Gossypium hirsutum L.).
Quantitative trait loci analysis of fiber quality traits using a random-mated recombinant inbred population in Upland cotton (Gossypium hirsutum L.).
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DOI：
10.1186/1471-2164-15-397
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发表时间：
2014-05-24
期刊：
影响因子：
4.4
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Fang DD;Jenkins JN;Deng DD;McCarty JC;Li P;Wu J
高地棉（Gossypium hirsutum L.）约占世界棉花产量的95%。改良高地棉花品种一直是世界范围内棉花育种计划的焦点。产量与纤维品质的负相关是棉花改良的障碍。随机交配提供了一种打破这种相关性的潜在方法。影响成纱质量的纤维品质性状包括长度、强力、成熟度、细度、伸长率、均匀度和颜色。在高地棉中定位稳定的纤维数量性状基因座（QTL）是利用分子标记辅助选择（MAS）改良棉花上级品质的基础。以11个不同的高地棉花品种为亲本，经过6轮随机交配和6代自交，构建了由550个重组自交系组成的随机交配重组自交系群体。550株RIL在美国密西西比州密西西比州一式三份种植两年，以获取纤维质量数据。通过对15538个SSR标记的筛选，在11个亲本中有2132个标记具有多态性。利用覆盖棉花基因组83%的1582个标记对275个重组自交系进行了基因分型。使用软件程序TASSEL分析标记-性状关联。在p < 0.01时，共检测到131个纤维QTL和37个QTL簇。这些QTL对短纤维含量和伸长率的综合表型变异的贡献率分别为62.3%和82.8%。利用270个SSR标记对其余275个重组自交系（第2组）进行了QTL定位。在第7和16染色体上观察到两个主要的QTL簇。将这131个QTL与已发表的QTL进行比较，发现其中77个是已发现的，另外54个是新发现的。本研究中使用的11个亲本代表了美国栽培棉的多样性遗传库，其中10个是优良的商业品种。本文报道的纤维QTL，特别是QTL簇可以很容易地在棉花育种计划中实施，以通过MAS策略改善纤维品质。一致QTL区域值得进一步研究，以更好地了解纤维发育的遗传和分子机制。本文的在线版本（doi：10.1186/1471-2164-15-397）包含补充材料，可供授权用户使用。
Upland cotton (Gossypium hirsutum L.) accounts for about 95% of world cotton production. Improving Upland cotton cultivars has been the focus of world-wide cotton breeding programs. Negative correlation between yield and fiber quality is an obstacle for cotton improvement. Random-mating provides a potential methodology to break this correlation. The suite of fiber quality traits that affect the yarn quality includes the length, strength, maturity, fineness, elongation, uniformity and color. Identification of stable fiber quantitative trait loci (QTL) in Upland cotton is essential in order to improve cotton cultivars with superior quality using marker-assisted selection (MAS) strategy. Using 11 diverse Upland cotton cultivars as parents, a random-mated recombinant inbred (RI) population consisting of 550 RI lines was developed after 6 cycles of random-mating and 6 generations of self-pollination. The 550 RILs were planted in triplicates for two years in Mississippi State, MS, USA to obtain fiber quality data. After screening 15538 simple sequence repeat (SSR) markers, 2132 were polymorphic among the 11 parents. One thousand five hundred eighty-two markers covering 83% of cotton genome were used to genotype 275 RILs (Set 1). The marker-trait associations were analyzed using the software program TASSEL. At p < 0.01, 131 fiber QTLs and 37 QTL clusters were identified. These QTLs were responsible for the combined phenotypic variance ranging from 62.3% for short fiber content to 82.8% for elongation. The other 275 RILs (Set 2) were analyzed using a subset of 270 SSR markers, and the QTLs were confirmed. Two major QTL clusters were observed on chromosomes 7 and 16. Comparison of these 131 QTLs with the previously published QTLs indicated that 77 were identified before, and 54 appeared novel. The 11 parents used in this study represent a diverse genetic pool of the US cultivated cotton, and 10 of them were elite commercial cultivars. The fiber QTLs, especially QTL clusters reported herein can be readily implemented in a cotton breeding program to improve fiber quality via MAS strategy. The consensus QTL regions warrant further investigation to better understand the genetics and molecular mechanisms underlying fiber development. The online version of this article (doi:10.1186/1471-2164-15-397) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Blenda A;Scheffler J;Scheffler B;Palmer M;Lacape JM;Yu JZ;Jesudurai C;Jung S;Muthukumar S;Yellambalase P;Ficklin S;Staton M;Eshelman R;Ulloa M;Saha S;Burr B;Liu S;Zhang T;Fang D;Pepper A;Kumpatla S;Jacobs J;Tomkins J;Cantrell R;Main D
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影响因子：
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影响因子：
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作者：
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