Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles.
Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles.
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社会隔离改变了雄性和雌性草原田鼠的行为、肠道-免疫-大脑轴和神经化学回路。
DOI：
10.1016/j.ynstr.2020.100278
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发表时间：
2020-11
影响因子：
5
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Donovan M;Mackey CS;Platt GN;Rounds J;Brown AN;Trickey DJ;Liu Y;Jones KM;Wang Z
缺乏社会支持或社会孤立可能会给人带来压力，导致一系列身心健康问题。越来越多的证据表明，肠道免疫脑轴的紊乱在社交隔离压力带来的负面后果中起着至关重要的作用。然而，其机制在很大程度上仍不清楚。社会一夫一妻制草原田鼠(Microtus Ochrogaster)已被证实是研究社会隔离对大脑和行为的负面影响的有用模型，但孤立的草原田鼠的肠道微生物群和中枢免疫系统如何改变仍是未知的。在这里，我们利用这种社交啮齿动物来研究社交隔离压力是如何改变肠道免疫脑轴和相关行为的。成年雄性和雌性草原田鼠(n=48)经历了社会隔离或与同性笼子(对照)同居6周。然后，检查他们的社交和焦虑样行为、神经元回路激活、神经化学表达、关键脑区的小胶质细胞增多症，以及隔离治疗后肠道微生物群的变化。社交孤立会增加类似焦虑的行为，并损害社会联系。隔离还会导致神经元激活、神经化学表达和小胶质细胞增多的性别和脑区特异性改变。此外，社交隔离导致肠道微生物群发生变化，这些微生物群与关键的大脑和行为指标相关。我们的数据表明，社会隔离以一种性别依赖的方式改变肠道免疫脑轴，肠道微生物、中央神经胶质细胞和神经化学系统可能在调节社会隔离的负面结果方面发挥关键的综合作用。社交孤立会增加类似焦虑的行为，并损害社会联系。隔离改变了大脑中神经元的激活和神经化学表达。隔离应激以特定脑区方式增加小胶质细胞。隔离压力也会导致肠道微生物群的改变。社交隔离以一种性别依赖的方式改变了肠道免疫脑轴。
The absence of social support, or social isolation, can be stressful, leading to a suite of physical and psychological health issues. Growing evidence suggests that disruption of the gut-immune-brain axis plays a crucial role in the negative outcomes seen from social isolation stress. However, the mechanisms remain largely unknown. The socially monogamous prairie vole (Microtus ochrogaster) has been validated as a useful model for studying negative effects of social isolation on the brain and behaviors, yet how the gut microbiome and central immune system are altered in isolated prairie voles are still unknown. Here, we utilized this social rodent to examine how social isolation stress alters the gut-immune-brain axis and relevant behaviors. Adult male and female prairie voles (n = 48 per sex) experienced social isolation or were cohoused with a same-sex cagemate (control) for six weeks. Thereafter, their social and anxiety-like behaviors, neuronal circuit activation, neurochemical expression, and microgliosis in key brain regions, as well as gut microbiome alterations from the isolation treatment were examined. Social isolation increased anxiety-like behaviors and impaired social affiliation. Isolation also resulted in sex- and brain region-specific alterations in neuronal activation, neurochemical expression, and microgliosis. Further, social isolation resulted in alterations to the gut microbiome that were correlated with key brain and behavioral measures. Our data suggest that social isolation alters the gut-immune-brain axis in a sex-dependent manner and that gut microbes, central glial cells, and neurochemical systems may play a critical, integrative role in mediating negative outcomes from social isolation. Social isolation increased anxiety-like behaviors and impaired social affiliation. Isolation altered neuronal activation and neurochemical expression in the brain. Isolation stress increased microgliosis in a brain region-specific manner. Isolation stress also resulted in alterations to the gut microbiome. Social isolation altered the gut-immune-brain axis in a sex-dependent manner.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.8
作者：
Clark, JS
通讯作者：
Clark, JS
影响因子：
2.7
作者：
Bahi, Amine
通讯作者：
Bahi, Amine
DOI：
10.1073/pnas.93.21.11980
发表时间：
1996-10-15
影响因子：
11.1
作者：
DeVries, AC;DeVries, MB;Carter, CS
通讯作者：
Carter, CS
影响因子：
24.8
作者：
Cacioppo JT;Cacioppo S;Capitanio JP;Cole SW
通讯作者：
Cole SW