Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls.
Differential expression of proteomics models of colorectal cancer, colorectal benign disease and healthy controls.
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结直肠癌、结直肠良性疾病和健康对照蛋白质组学模型的差异表达
DOI：
10.1186/1477-5956-8-16
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发表时间：
2010-03-25
期刊：
影响因子：
2
通讯作者：
Liu JR
中科院分区：
文献类型：
--
作者：
Liu M;Li CF;Chen HS;Lin LQ;Zhang CP;Zhao JL;Liu Y;Zhang SJ;Jin JC;Wang L;Liu JR
研究背景结直肠癌(CRC)诊断多为晚期，预后较差。蛋白质组学的超灵敏分析技术可以在肿瘤可触及之前检测到分子变化。表面增强激光解吸电离飞行时间质谱仪(SELDI-TOF-MS)是近年来发展起来的一种新的蛋白质分离评价技术。蛋白质芯片已经确立了肿瘤蛋白在血清标本中的表达，成为新发现的肿瘤诊断标记物。本研究的目的是在结直肠癌、结直肠良性疾病(CBD)和健康对照组之间寻找新的诊断标记物。采用SELDI-TOF-MS结合蛋白质芯片生物信息学分析技术，检测患者和对照组血清中蛋白质质量峰的表达。采用WCX2蛋白芯片对52例结直肠癌患者、27例结直肠良性疾病患者和21例健康体检者的血清标本进行SELDI-TOF-MS检测。这些模型结合3个蛋白质质量峰来区分结直肠癌、CBD和健康对照。结论SELDI-TOF-MS是一种辅助诊断结直肠癌的有用工具，尤其是在结直肠癌的早期阶段。然而，对差异显著的蛋白质的鉴定还需要进一步的研究。
BackgroundColorectal cancer (CRC) is often diagnosed at a late stage with concomitant poor prognosis. The hypersensitive analytical technique of proteomics can detect molecular changes before the tumor is palpable. The surface-enhanced laser desorption/ionization-time of flight-mass spectra (SELDI-TOF-MS) is a newly-developed technique of evaluating protein separation in recent years. The protein chips have established the expression of tumor protein in the serum specimens and become the newly discovered markers for tumor diagnosis. The objective of this study was to find new markers of the diagnosis among groups of CRC, colorectal benign diseases (CBD) and healthy controls. The assay of SELDI-TOF-MS with analytical technique of protein-chip bioinformatics was used to detect the expression of protein mass peaks in the sera of patients or controls. One hundred serum samples, including 52 cases of colorectal cancer, 27 cases of colorectal benign disease, and 21 cases of healthy controls, were examined by SELDI-TOF-MS with WCX2 protein-chips.ResultsThe diagnostic models (I, II and III) were setup by analyzed the data and sieved markers using Ciphergen - Protein-Chip-Software 5.1. These models were combined with 3 protein mass peaks to discriminate CRC, CBD, and healthy controls. The accuracy, the sensitivity and the particularity of cross verification of these models are all highly over 80%.ConclusionsThe SELDI-TOF-MS is a useful tool to help diagnose colorectal cancer, especially during the early stage. However, identification of the significantly differentiated proteins needs further study.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
癌组学对癌症生物标志物发现的贡献。
影响因子：
37.3
作者：
Cho, William C S
通讯作者：
Cho, William C S
影响因子：
2.8
作者：
BARILLARI, P;RAMACCIATO, G;FEGIZ, G
通讯作者：
FEGIZ, G
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
2
作者：
Voortman J;Pham TV;Knol JC;Giaccone G;Jimenez CR
通讯作者：
Jimenez CR