In vivo time-lapse imaging of cell proliferation and differentiation in the optic tectum of Xenopus laevis tadpoles.
In vivo time-lapse imaging of cell proliferation and differentiation in the optic tectum of Xenopus laevis tadpoles.
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DOI：
10.1002/cne.22795
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发表时间：
2012-02-01
影响因子：
2.5
通讯作者：
Cline, Hollis T.
中科院分区：
文献类型：
--
作者：
Bestman, Jennifer E.;Lee-Osbourne, Jane;Cline, Hollis T.
关键词：
我们分析了神经祖细胞在非洲爪蟾蝌蚪发育中的中枢神经系统的功能，使用在体内的时间推移共聚焦显微镜收集图像，通过顶盖在2至24小时的时间间隔超过3天。基于内源性Sox 2转录因子的表达，用荧光蛋白报告基因标记神经前体细胞。利用这种结构，我们鉴定了Sox 2表达细胞为放射状神经胶质细胞，并作为发育中的顶盖中产生神经元和其他放射状神经胶质细胞的祖细胞库的组成部分。单个放射状神经胶质细胞及其后代的谱系分析表明，不到10%的放射状神经胶质细胞进行对称分裂，产生两个放射状神经胶质细胞，而大多数放射状神经胶质细胞不对称分裂，产生神经元和放射状神经胶质细胞。延时成像显示放射状胶质细胞直接分化为神经元。虽然放射状胶质细胞可以引导轴突，因为他们导航到表层顶盖，我们没有发现任何证据表明，放射状胶质细胞功能作为一个支架的神经元迁移在顶盖发育的早期阶段。在三天内，标记细胞的数量增加了20%，放射状胶质细胞的分数下降，神经元后代的比例增加到约60%的标记细胞。短期视觉增强的蝌蚪产生了更多的神经元，细胞增殖相应减少。总之，这些结果表明，放射状神经胶质细胞是神经祖细胞在发展中的视顶盖，并揭示视觉经验增加的比例，在一个完整的动物产生的神经元。
We analyzed the function of neural progenitors in the developing CNS of Xenopus laevis tadpoles using in vivo time-lapse confocal microscopy to collect images through the tectum at intervals of 2 to 24 hours over 3 days. Neural progenitor cells were labeled with fluorescent protein reporters based on expression of endogenous Sox2 transcription factor. With this construct, we identified Sox2-expressing cells as radial glia and as a component of the progenitor pool of cells in the developing tectum that gives rise to neurons and other radial glia. Lineage analysis of individual radial glia and their progeny demonstrated that less than 10% of radial glia undergo symmetric divisions resulting in two radial glia, while the majority of radial glia divide asymmetrically to generate neurons and radial glia. Time-lapse imaging revealed the direct differentiation of radial glia into neurons. Although radial glia may guide axons as they navigate to superficial tectum, we find no evidence that radial glia function as a scaffold for neuronal migration at early stages of tectal development. Over three days, the number of labeled cells increased 20%, as the fraction of radial glia dropped and the proportion of neuronal progeny increased to approximately 60% of the labeled cells. Tadpoles provided with short-term visual enhancement generated significantly more neurons, with a corresponding decrease in cell proliferation. Together these results demonstrate that radial glial cells are neural progenitors in the developing optic tectum and reveal that visual experience increases the proportion of neurons generated in an intact animal.
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影响因子：
4.6
作者：
Caron, Sophie J. C.;Prober, David;Choy, Margaret;Schier, Alexander F.
通讯作者：
Schier, Alexander F.
影响因子：
2.5
作者：
Dong, Wei;Lee, Ryan H.;Aizenman, Carlos D.
通讯作者：
Aizenman, Carlos D.
影响因子：
2.5
作者：
Aizenman, Carlos D.;Cline, Hollis T.
通讯作者：
Cline, Hollis T.
影响因子：
2.5
作者：
EDWARDS, MA;CAVINESS, VS;SCHNEIDER, GE
通讯作者：
SCHNEIDER, GE
影响因子：
13.9
作者：
Barnes AP;Polleux F
通讯作者：
Polleux F