Pregnant sows immunized with Cryptosporidium parvum significantly reduced infection in newborn piglets challenged with C. parvum but not with C. hominis.
Pregnant sows immunized with Cryptosporidium parvum significantly reduced infection in newborn piglets challenged with C. parvum but not with C. hominis.
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用微小隐孢子虫免疫妊娠母猪可显著降低新生仔猪感染C. parvum，但与C.人类
DOI：
10.1371/journal.pntd.0010690
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发表时间：
2022-07
影响因子：
3.8
通讯作者：
中科院分区：
文献类型：
--
作者：
仔猪是研究人隐孢子虫与隐孢子虫免疫原性关系的唯一模型。小虫是导致人类腹泻的物种。尽管在抗原性上难以区分，并且它们之间具有高度的遗传同源性，但它们在活动性感染后仅具有中度交叉保护性。在这里，我们研究了在怀孕期间用C.小的出生后，用C. parvum或C. 5天大的人类用C.小隐孢子虫感染率显著降低，而攻毒仔猪感染率显著降低。尽管C.血清和初乳IgG和伊加抗体。我们将这些数据添加到早期的研究中，在这些研究中，我们描述了感染源性免疫提供了部分交叉保护。总之，似乎为了充分保护，针对人类隐孢子虫病的疫苗必须含有来自两个物种的抗原成分。隐孢子虫病是一种引起腹泻和脱水的寄生虫感染，在20世纪80年代随着艾滋病大流行而成为全球肠道病原体。隐孢子虫感染已发展成为低收入至中等收入国家2岁以下儿童发病和死亡的一个严重原因。鉴于这些发展，迫切需要一种有效的人类疫苗。C. hominis和C. parvum是隐孢子虫属的两个种，C.人型比C.小的在本研究中，C.检查妊娠期间的小孢子。将新生仔猪分为4组：2组为C. parvum和2个对照组来自未免疫母猪。一个免疫组和一个对照组感染C.第二免疫组和第二对照组感染C.人类新生仔猪同样和完全保护免受腹泻病，无论它们喂养的母猪是否免疫。结果证实，母畜的初乳和乳汁传递非特异性疾病预防元素，以充分保护免受疾病，而被动获得的特异性抗体大大降低了感染的程度。
The piglet is the only model to investigate the immunogenic relationship between Cryptosporidium hominis and C. parvum, the species responsible for diarrhea in humans. Despite being indistinguishable antigenically, and high genetic homology between them, they are only moderately cross protective after an active infection. Here we examined the degree of passive protection conferred to piglets suckling sows immunized during pregnancy with C. parvum. After birth suckling piglets were challenged orally with either C. parvum or C. hominis at age 5 days. Animals challenged with C. parvum had significant reduction of infection rate, while piglets challenged with C. hominis showed no reduction despite high C. parvum serum and colostrum IgG and IgA antibody. We add these data to earlier studies where we described that infection derived immunity provides partial cross-protection. Together, it appears that for full protection, vaccines against human cryptosporidiosis must contain antigenic elements derived from both species. Cryptosporidiosis, a parasitic infection causing diarrhea and dehydration, emerged as a global enteric pathogen in the 1980s with the AIDS pandemic. Cryptosporidium infections have evolved to become a serious cause of morbidity and mortality in children under the age of 2 years in low to middle income countries. Given these developments, there is a critical need for an effective human vaccine. C. hominis and C. parvum are two Cryptosporidium species, with C. hominis being more common and more infectious than C. parvum. In this study, passive protection conferred to piglets suckling sows immunized with C. parvum during pregnancy was examined. Newborn piglets were separated into 4 groups: 2 groups from sows immunized with C. parvum and 2 control groups from unimmunized sows. One immunized group and one control group were infected with C. parvum, and the second immunized group and the second control group were infected with C. hominis. Newborn piglets were equally and fully protected against diarrheal disease regardless of whether the sow off which they fed was immunized. The results confirm that the dam’s colostrum and milk convey non-specific disease-preventing elements for full protection from disease, while passively acquired specific antibody reduces considerably the extent of infection.
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影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
影响因子：
2.6
作者：
Martín-Gómez, S;Alvarez-Sánchez, MA;Rojo-Vázquez, F
通讯作者：
Rojo-Vázquez, F
影响因子：
7.1
作者：
Molbak, K;Andersen, M;daSilva, APJ
通讯作者：
daSilva, APJ
DOI：
10.1093/cid/cis761
发表时间：
2012-12
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Levine MM;Kotloff KL;Nataro JP;Muhsen K
通讯作者：
Muhsen K
DOI：
10.4269/ajtmh.2005.73.921
发表时间：
2005-11-01
影响因子：
3.3
作者：
Tumwine, JK;Kekitiinwa, A;Tzipori, S
通讯作者：
Tzipori, S