Manipulating the Xenopus genome with transposable elements.
Manipulating the Xenopus genome with transposable elements.
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DOI：
10.1186/gb-2007-8-s1-s11
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Mead PE
中科院分区：
文献类型：
--
作者：
Yergeau DA;Mead PE
两栖动物胚胎发生的研究为脊椎动物的发育机制提供了重要的线索。几十年来，非洲爪蟾一直是脊椎动物细胞生物学和发育的重要模型。由于该物种的基因组具有四倍体的性质，且世代时间较长，因此对该物种的遗传研究并不实用。最近，一种与之密切相关的青蛙，即热带爪蟾，被提出作为一个替代系统;它拥有X的所有物理特征。Laev是一个有用的模型，但具有二倍体基因组和短世代时间的优点。该动物遗传资源的快速积累和中试诱变筛选的成功有助于推动该模型系统向前发展。转座因子将为操纵青蛙基因组提供宝贵的工具。这些整合系统非常适合于青蛙的转基因和插入诱变策略。高繁殖力的青蛙结合再动员转座子转基因整合到青蛙基因组的能力，将允许大规模的插入诱变筛选在实验室进行适度的饲养能力。
The study of amphibian embryogenesis has provided important insight into the mechanisms of vertebrate development. The frog Xenopus laevis has been an important model of vertebrate cell biology and development for many decades. Genetic studies in this organism are not practical because of the tetraploid nature of the genome and the long generation time of this species. Recently, a closely related frog, namely Xenopus tropicalis, has been proposed as an alternative system; it shares all of the physical characteristics that make X. laevis a useful model but has the advantage of a diploid genome and short generation time. The rapid accumulation of genetic resources for this animal and the success of pilot mutagenesis screens have helped propel this model system forward. Transposable elements will provide invaluable tools for manipulating the frog genome. These integration systems are ideally suited to transgenesis and insertional mutagenesis strategies in the frog. The high fecundity of the frog combined with the ability to remobilize transposon transgenes integrated into frog genome will allow large-scale insertional mutagenesis screens to be performed in laboratories with modest husbandry capacities.
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