Issue highlights--November 2014.
Issue highlights--November 2014.
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问题要点——2014 年 11 月。
DOI：
10.1002/cyto.b.21186
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
McCoyJr,JPhilip
中科院分区：
文献类型：
--
作者：
McCoyJr,JPhilip
本期《细胞计量学B部分：临床细胞计量学》包含了许多关于各种主题的激动人心的文章。其中包括Peijers和他的同事的一篇关于免疫表型协调的文章。免疫表型的协调和标准化都是细胞学家非常感兴趣的话题。虽然标准化要求严格遵守公认的实践或标记集，但协调允许更大的灵活性，同时仍可实现高度一致的结果。在过去的几年里，各种临床分析的标准化一直是该杂志几篇关键手稿的主题(1-3)。例如，Sutherland和他的同事(1)以及Fletcher和他的同事(2)提出了对阵发性睡眠性血红蛋白尿症(PNH)克隆检测标准化的努力。近年来，协调流入细胞术一直是高可见度论文的主题，例如Maecker和他的同事的论文(4，5)，因为人们越来越认识到实验室之间需要高度一致，同时仍允许个别实验室在进行分析时具有一定的灵活性。在这一期中，Preijers和他的同事(6)将替代抗体克隆和细胞仪的使用与原始标准化方案中使用的进行了比较(7)。通过证明标准试剂和他们自己的试剂之间的高度可比性，Preijers等人使这项协议在特定品牌的试剂或仪器的使用方面更加不可知，从而协调了潜在更广泛的实验室组之间的数据收集。此外，在这一期中，Krishnan和他的同事密切关注了抗凝剂、年龄和性别等分析变量在测量健康捐赠者外周循环中的细胞因子和趋化因子(8)。这些变量越来越多地被认为对多重珠状细胞因子分析(9)和免疫表型分析(10)的数据有显著影响。在目前的研究中，Krishnan和他的同事在使用23-plex珠阵列法测量各种细胞因子和趋化因子方面显示出显著的抗凝血剂和年龄相关性差异。从这篇论文中得到的一个关键的“带回家”的信息是细胞因子测量的临床方案的适当设计和实施。诊所必须注意只收集方案中规定的一种抗凝剂中的所有标本，并且永远不能用血清代替血浆，反之亦然。这样做可能会导致数据中出现伪影，并导致研究中的虚假结论。在几种细胞因子水平上观察到的与年龄相关的差异表明，需要仔细匹配任何对照队列的年龄与研究对象。
This issue of Cytometry Part B: Clinical Cytometry contains a number of exciting articles on a wide variety of topics. Included among these is an article by Peijers and colleagues concerning the harmonization of immunophenotyping. Both harmonization and standardization of immunophenotyping are topics of keen interest to cytometrists. Whereas standardization requires strict adherence to an accepted practice or marker set, harmonization permits more flexibility while still achieving highly consistent results. Standardization of various clinical assays has been the topic of several key manuscripts in this journal over the past several years (1–3). For example, efforts towards the standardization of detection of clones in paroxysmal nocturnal hemoglobinuria (PNH) have been presented by Sutherland and colleagues (1) as well as by Fletcher and coworkers (2). In recent years harmonization inflow cytometry has been the subject of high visibility papers such as those by Maecker and colleagues (4, 5) as it has been increasingly recognized that there needs to be a high degree of consistency among laboratories while still permitting a certain amount of flexibility in the performance of assays by individual laboratories. In this issue, Preijers and coworkers (6) compare the use of alternative antibody clones and cytometers to those used in the original standardized protocol (7). By demonstrating a high degree of comparability between the standard reagents and their own, Preijers et al have made this protocol more ‘agnostic’about the use of a particular brand of reagents or instrument, and thus harmonizing data collection among a potentially wider group of laboratories.Also in this issue, Krishnan and coworkers take a close look at analytical variables such as anticoagulants, age, and gender in the measurement of cytokines and chemokines from the peripheral circulation of healthy donors (8). Increasingly, these variables are being recognized as significantly impacting data from both multiplex bead cytokine assays (9) as well as immunophenotyping (10). In the current study Krishnan and colleagues show profound anti-coagulant and age-dependent differences in the measurement of various cytokines and chemokines using a 23-plex bead array assay. A key ‘take home’message from this paper is the proper design and implementation of clinical protocols for cytokine measurements. The clinic must take care to collect all specimens in only the one anticoagulant specified in the protocol and never substitute serum for plasma, or vice versa. Doing so could lead to artifacts within the data and lead to spurious conclusions in the study. The agerelated differences observed in the levels of several cytokines indicate the need to carefully match the ages of any control cohort to the subjects of the study.
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影响因子：
4.3
作者：
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影响因子：
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作者：
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DOI：
10.1093/jnci/46.4.831
发表时间：
1971
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
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通讯作者：
H. Rapp
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影响因子：
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作者：
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通讯作者：
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