A C-type lectin in saliva of Aedes albopictus (Diptera: Culicidae) bind and agglutinate microorganisms with broad spectrum.
A C-type lectin in saliva of Aedes albopictus (Diptera: Culicidae) bind and agglutinate microorganisms with broad spectrum.
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DOI：
10.1093/jisesa/iead043
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发表时间：
2023-07-01
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影响因子：
--
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中科院分区：
文献类型：
--
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通过复杂的唾液混合物，蚊子可以干预免疫反应，并有助于传播几种导致致命人类疾病的病毒。一些c型凝集素（ctl）被报道为模式识别受体，可以抵抗或促进病原体的入侵。在这里，我们研究了携带单一碳水化合物识别结构域（CRD）和WND/KPD基序的白纹伊蚊CTL （Aalb_CTL2）的表达谱和凝集功能。结果表明，Aalb_CTL2在蚊子唾液腺中特异性表达，且不受血供诱导。重组蛋白Aalb_CTL2 （rAalb_CTL2）能在钙存在下凝集小鼠红细胞，其凝集活性可被EDTA抑制。rAalb_CTL2还显示出与d -甘露糖、d -半乳糖、d -葡萄糖和麦芽糖的糖结合能力。结果表明，rAalb_CTL2能在体外以钙依赖的方式结合和凝集革兰氏阳性菌金黄色葡萄球菌和枯草芽孢杆菌，革兰氏阴性菌大肠杆菌和铜绿假单胞菌，以及真菌白色念珠菌。而rAalb_CTL2在THP-1和BHK-21细胞系中不能促进DENV-2的复制。这些发现揭示了Aalb_CTL2可能参与了蚊子抵抗糖和血中微生物繁殖的先天免疫，从而帮助蚊子在多变的自然环境中生存。
Via complex salivary mixture, mosquitos can intervene immune response and be helpful to transmit several viruses causing deadly human diseases. Some C-type lectins (CTLs) of mosquito have been reported to be pattern recognition receptor to either resist or promote pathogen invading. Here, we investigated the expression profile and agglutination function of an Aedes albopictus CTL (Aalb_CTL2) carrying a single carbohydrate-recognition domain (CRD) and WND/KPD motifs. The results showed that Aalb_CTL2 was found to be specifically expressed in mosquito saliva gland and its expression was not induced by blood-feeding. The recombinant Aalb_CTL2 (rAalb_CTL2) could agglutinate mouse erythrocytes in the presence of calcium and the agglutinating activity could be inhibited by EDTA. rAalb_CTL2 also displayed the sugar binding ability to D-mannose, D-galactose, D-glucose, and maltose. Furthermore, it was demonstrated that rAalb_CTL2 could bind and agglutinate Gram positive bacteria Staphylococcus aureus and Bacillus subtilis, Gram negative bacteria Escherichia coli and Pseudomonas aeruginosa, as well as fungus Candida albicans in vitro in a calcium dependent manner. However, rAalb_CTL2 could not promote type 2 dengue virus (DENV-2) replication in THP-1 and BHK-21 cell lines. These findings uncover that Aalb_CTL2 might be involved in the innate immunity of mosquito to resist microorganism multiplication in sugar and blood meals to help mosquito survive in the varied natural environment.
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影响因子：
5.5
作者：
Martin-Martin I;Smith LB;Chagas AC;Sá-Nunes A;Shrivastava G;Valenzuela-Leon PC;Calvo E
通讯作者：
Calvo E
DOI：
10.1126/science.1193162
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bartholomay LC;Waterhouse RM;Mayhew GF;Campbell CL;Michel K;Zou Z;Ramirez JL;Das S;Alvarez K;Arensburger P;Bryant B;Chapman SB;Dong Y;Erickson SM;Karunaratne SH;Kokoza V;Kodira CD;Pignatelli P;Shin SW;Vanlandingham DL;Atkinson PW;Birren B;Christophides GK;Clem RJ;Hemingway J;Higgs S;Megy K;Ranson H;Zdobnov EM;Raikhel AS;Christensen BM;Dimopoulos G;Muskavitch MA
通讯作者：
Muskavitch MA
影响因子：
3.8
作者：
Ribeiro, JMC;Charlab, R;Valenzuela, JG
通讯作者：
Valenzuela, JG
用于预防登革热的蚊子 C 型凝集素传播阻断抗体
影响因子：
6.7
作者：
Liu Y;Zhang F;Liu J;Xiao X;Zhang S;Qin C;Xiang Y;Wang P;Cheng G
通讯作者：
Cheng G
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC