Estrogen via estrogen receptor beta partially inhibits mandibular condylar cartilage growth.
Estrogen via estrogen receptor beta partially inhibits mandibular condylar cartilage growth.
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DOI：
10.1016/j.joca.2014.07.003
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发表时间：
2014-11
影响因子：
7
通讯作者：
Wadhwa, S.
中科院分区：
文献类型：
--
作者：
Chen, J.;Kamiya, Y.;Polur, I.;Xu, M.;Choi, T.;Kalajzic, Z.;Drissi, H.;Wadhwa, S.
颞下颌关节疾病主要影响女性，提示女性激素在疾病过程中的作用。然而，雌激素受体（ER）信号在调节下颌髁软骨生长中的作用知之甚少。因此，本研究的目的是研究雌激素水平改变对WT和ER β KO小鼠下颌髁软骨的影响。21日龄雌性WT （n=37）和ER β KO小鼠（n=36）分别接受假手术或卵巢切除，并给予安慰剂或雌二醇治疗。采用组织形态学法评估下颌髁突软骨，采用双EdU/BrdU标记法分析其增殖情况，并检测软骨细胞成熟标志物的基因和蛋白表达。在WT小鼠中，与假手术小鼠相比，卵巢切除术导致下颌髁软骨细胞数量显著增加，Sox9表达显著增加，增殖显著增加。相比之下，卵巢切除术在ER β KO小鼠中没有引起任何这些影响。与接受安慰剂治疗的去卵巢小鼠相比，经雌激素替代治疗的去卵巢WT小鼠ER α表达显著降低，Sost表达显著增加。在切除卵巢的ER β KO小鼠中，雌激素替代治疗导致Col2表达显著增加，ER α表达无变化，Sost表达显著增加。雌激素通过ER β抑制细胞增殖和ER α的表达，而不依赖ER β的雌激素诱导Col2和Sost的表达。
Temporomandibular joint diseases predominantly afflict women, suggesting a role for female hormones in the disease process. However, little is known about the role of estrogen receptor (ER) signaling in regulating mandibular condylar cartilage growth. Therefore, the goal of this study was to examine the effects of altered estrogen levels on the mandibular condylar cartilage in WT and ER beta KO mice. 21-day-old female WT (n=37) and ER beta KO mice (n=36) were either sham operated or ovariectomized, and treated with either placebo or estradiol. The mandibular condylar cartilage was evaluated by histomorphometry, proliferation was analyzed by double EdU/BrdU labeling, and assays on gene and protein expression of chondrocyte maturation markers were performed. In WT mice, ovariectomy caused a significant increase in mandibular condylar cartilage cell numbers, a significant increase in Sox9 expression and a significant increase in proliferation compared with sham operated WT mice. In contrast, ovariectomy did not cause any of these effects in the ER beta KO mice. Estrogen replacement treatment in ovariectomized WT mice caused a significant decrease in ER alpha expression and a significant increase in Sost expression compared with ovariectomized mice treated with placebo. Estrogen replacement treatment in ovariectomized ER beta KO mice caused a significant increase in Col2 expression, no change in ER alpha expression, and a significant increase in Sost expression. Estrogen via ER beta inhibits proliferation and ER alpha expression while estrogen independent of ER beta induces Col2 and Sost expression.
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DOI：
10.1016/j.jpain.2011.08.001
发表时间：
2011-11
期刊：
The journal of pain
影响因子：
--
作者：
Slade GD;Bair E;By K;Mulkey F;Baraian C;Rothwell R;Reynolds M;Miller V;Gonzalez Y;Gordon S;Ribeiro-Dasilva M;Lim PF;Greenspan JD;Dubner R;Fillingim RB;Diatchenko L;Maixner W;Dampier D;Knott C;Ohrbach R
通讯作者：
Ohrbach R
DOI：
10.1016/j.oooo.2013.04.019
发表时间：
2013-08-01
影响因子：
2.9
作者：
Lamot, Urska;Strojan, Primoz;Popovic, Katarina Surlan
通讯作者：
Popovic, Katarina Surlan
影响因子：
3.3
作者：
Schmitter, M.;Essig, M.;Rammelsberg, P.
通讯作者：
Rammelsberg, P.
影响因子：
1.9
作者：
Okuda, T;Yasuoka, T;Oka, N
通讯作者：
Oka, N
影响因子：
1.9
作者：
Luder, HU
通讯作者：
Luder, HU