Genetic and molecular characterization of submergence response identifies Subtol6 as a major submergence tolerance locus in maize.
Genetic and molecular characterization of submergence response identifies Subtol6 as a major submergence tolerance locus in maize.
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DOI：
10.1371/journal.pone.0120385
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Walia H
中科院分区：
文献类型：
--
作者：
Campbell MT;Proctor CA;Dou Y;Schmitz AJ;Phansak P;Kruger GR;Zhang C;Walia H
玉米对短期水淹和淹没高度敏感。早季淹水会降低玉米幼苗的发芽率、存活率和生长速率。本研究旨在通过对不同基因型的转录谱分析和连锁分析，揭示玉米耐淹性的遗传变异，阐明耐淹性的遗传基础。筛选了一组不同的玉米巢式关联作图（NAM）建立系，并鉴定了两个高耐性（Mo18W和M162W）和敏感（B97和B73）基因型。与敏感品系相比，耐受品系表现出延迟衰老和较低的氧化应激水平。对这些近交系进行转录组分析，以提供对淹没的分子响应的基因组水平的见解。耐受线有较高的转录丰度的几个发酵相关的基因和一个未注释的焦磷酸依赖果糖-6-磷酸1-磷酸转移酶基因在淹没。一个共表达网络丰富的CBF（C-REPEAT/DRE结合因子：C-REPEAT/DRE结合因子）基因，诱导淹没在所有四个自交系，但更多地激活耐受Mo18 W。对Mo18W和B73的重组自交系（RIL）群体进行耐淹性筛选。一个名为Subtol6的主效QTL被定位到第6号染色体上，解释了RIL群体内22%的表型变异。基于在B73和Mo18W中观察到的对比表达模式，我们确定了Subtol6的两个候选基因（血红蛋白2和RAV1）。本研究中确定的耐受性来源（Subtol6）可用于提高洪水事件期间的存活率，预计随着气候变化频率增加。
Maize is highly sensitive to short term flooding and submergence. Early season flooding reduces germination, survival and growth rate of maize seedlings. We aimed to discover genetic variation for submergence tolerance in maize and elucidate the genetic basis of submergence tolerance through transcriptional profiling and linkage analysis of contrasting genotypes. A diverse set of maize nested association mapping (NAM) founder lines were screened, and two highly tolerant (Mo18W and M162W) and sensitive (B97 and B73) genotypes were identified. Tolerant lines exhibited delayed senescence and lower oxidative stress levels compared to sensitive lines. Transcriptome analysis was performed on these inbreds to provide genome level insights into the molecular responses to submergence. Tolerant lines had higher transcript abundance of several fermentation-related genes and an unannotated Pyrophosphate-Dependent Fructose-6-Phosphate 1-Phosphotransferase gene during submergence. A coexpression network enriched for CBF (C-REPEAT/DRE BINDING FACTOR: C-REPEAT/DRE BINDING FACTOR) genes, was induced by submergence in all four inbreds, but was more activated in the tolerant Mo18W. A recombinant inbred line (RIL) population derived from Mo18W and B73 was screened for submergence tolerance. A major QTL named Subtol6 was mapped to chromosome 6 that explains 22% of the phenotypic variation within the RIL population. We identified two candidate genes (HEMOGLOBIN2 and RAV1) underlying Subtol6 based on contrasting expression patterns observed in B73 and Mo18W. Sources of tolerance identified in this study (Subtol6) can be useful to increase survival rate during flooding events that are predicted to increase in frequency with climate change.
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