Quantitative Characterization of the Toxicities of Cd-Ni and Cd-Cr Binary Mixtures Using Combination Index Method.

Quantitative Characterization of the Toxicities of Cd-Ni and Cd-Cr Binary Mixtures Using Combination Index Method.
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使用组合指数法定量表征 Cd-Ni 和 Cd-Cr 二元混合物的毒性

DOI:
10.1155/2016/4158451
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发表时间:
2016
影响因子:
--
通讯作者:
Liang Y
Liang Y
中科院分区:
生物学3区
文献类型:
--
作者:
Mo L;Zheng M;Qin M;Zhang X;Liu J;Qin L;Zeng H;Liang Y

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采用直接均分射线设计方法,构建了Cd-Ni和Cd-Cr二元混合物。采用微板毒性分析法研究了单一物质及Cd-Ni、Cd-Cr混合物对蛋白核小球藻和羊角月牙藻的毒性。用浓度加和模型分析了混合物的互作毒性。此外,提出了组合指数法(CI),并将其用于定量表征Cd-Ni和Cd-Cr二元混合物的毒性,得到与CA模型预测结果的偏离程度,即相互作用的毒性强度。结果表明,20种二元复配物中,大部分复配物具有增效作用,只有Cd-Cr-R4和Cd-Cr-R5复配物具有较高的拮抗作用。蛋白核基于置信区间,CI可以比较不同效应水平下混合物的相互作用强度。该表征方法适用于分析具有复杂相互作用的二元混合物。
Direct equipartition ray design was used to construct Cd-Ni and Cd-Cr binary mixtures. Microplate toxicity analysis was used to evaluate the toxicity of individual substance and the Cd-Ni and Cd-Cr mixtures on Chlorella pyrenoidosa and Selenastrum capricornutum. The interacting toxicity of the mixture was analyzed with concentration addition (CA) model. In addition, combination index method (CI) was proposed and used to quantitatively characterize the toxicity of the binary mixtures of Cd-Ni and Cd-Cr observed in experiment and find the degree of deviation from the predicted outcome of the CA model, that is, the intensity of interacting toxicity. Results indicate that most of the 20 binary mixtures exhibit enhancing and synergistic effect, and only Cd-Cr-R4 and Cd-Cr-R5 mixtures have relatively high antagonistic effects against C. pyrenoidosa. Based on confidence interval, CI can compare the intensities of interaction of the mixtures under varying levels of effect. The characterization methods are applicable for analyzing binary mixture with complex interaction.
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