Glucose increases synaptic transmission from vagal afferent central nerve terminals via modulation of 5-HT3 receptors.
Glucose increases synaptic transmission from vagal afferent central nerve terminals via modulation of 5-HT3 receptors.
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葡萄糖通过调节 5-HT3 受体来增加迷走神经传入中枢神经末梢的突触传递。
DOI：
10.1152/ajpgi.90288.2008
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Browning,KirsteenN
中科院分区：
文献类型：
--
作者：
Wan,Shuxia;Browning,KirsteenN
急性高血糖对迷走神经介导的胃肠功能有深远的影响。我们最近报道迷走神经传入神经元中央终末释放谷氨酸与细胞外葡萄糖浓度直接相关。本研究旨在验证迷走神经传入神经末梢上存在的5-HT3受体参与这种葡萄糖依赖的谷氨酸能突触传递调节的假说。全细胞膜片钳记录大鼠脑干薄片孤束核(NTS)神经元。5-HT3受体选择性拮抗剂恩丹西酮以浓度依赖的方式抑制自发和诱发的谷氨酸释放。胞外葡萄糖浓度的变化导致恩丹西酮介导的谷氨酸释放抑制的平行变化。5-羟色胺摄取抑制剂芬氟拉明可模拟细胞外葡萄糖浓度引起的兴奋性突触传递的变化。这些结果表明，葡萄糖通过作用于紧张性5-HT3受体而改变了大鼠脑干内兴奋性突触传递，迷走神经传入神经末梢上5-HT3受体的数量与细胞外葡萄糖浓度呈正相关。这些数据表明，在迷走神经传入神经末梢和NTS神经元之间的突触连接上存在的5-HT3受体是葡萄糖调节迷走神经反射的有力候选部位之一。
Acute hyperglycemia has profound effects on vagally mediated gastrointestinal functions. We have reported recently that the release of glutamate from the central terminals of vagal afferent neurons is correlated directly with the extracellular glucose concentration. The present study was designed to test the hypothesis that 5-HT3receptors present on vagal afferent nerve terminals are involved in this glucose-dependent modulation of glutamatergic synaptic transmission. Whole-cell patch-clamp recordings were made from neurons of the nucleus tractus solitarius (NTS) in thin rat brainstem slices. Spontaneous and evoked glutamate release was decreased in a concentration-dependent manner by the 5-HT3receptor selective antagonist, ondansetron. Alterations in the extracellular glucose concentration induced parallel shifts in the ondansetron-mediated inhibition of glutamate release. The changes in excitatory synaptic transmission induced by extracellular glucose concentration were mimicked by the serotonin uptake inhibitor, fenfluramine. These data suggest that glucose alters excitatory synaptic transmission within the rat brainstem via actions on tonically active 5-HT3receptors, and the number of 5-HT3receptors on vagal afferent nerve terminals is positively correlated with the extracellular glucose concentration. These data indicate that the 5-HT3receptors present on synaptic connections between vagal afferent nerve terminals and NTS neurons are a strong candidate for consideration as one of the sites where glucose acts to modulate vagovagal reflexes.
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DOI：
10.1152/ajpgi.2001.281.4.g916
发表时间：
2001-10
期刊：
American journal of physiology. Gastrointestinal and liver physiology
影响因子：
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DOI：
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发表时间：
1996
期刊：
The Journal of physiology
影响因子：
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作者：
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通讯作者：
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DOI：
10.1152/ajpregu.1988.255.2.r200
发表时间：
1988-08
期刊：
The American journal of physiology
影响因子：
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