Anopheles ecology, genetics and malaria transmission in northern Cambodia.
Anopheles ecology, genetics and malaria transmission in northern Cambodia.
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DOI：
10.1038/s41598-021-85628-1
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发表时间：
2021-03-19
影响因子：
4.6
通讯作者：
Witkowski B
中科院分区：
文献类型：
--
作者：
Vantaux A;Riehle MM;Piv E;Farley EJ;Chy S;Kim S;Corbett AG;Fehrman RL;Pepey A;Eiglmeier K;Lek D;Siv S;Mueller I;Vernick KD;Witkowski B
在大湄公河次区域，疟疾病例已显着减少，但人们对造成残留疟疾传播的媒介或机制知之甚少。我们分析了在雨季和旱季从柬埔寨四个生态环境（村庄、村庄附近的森林地区、橡胶树种植园和林地）收集的总共 3920 只按蚊。使用气味诱饵陷阱，所有地点的总样本中有 81% 是在牛诱饵陷阱中收集的，尽管人类诱饵陷阱吸引的样本中有 67% 是在森林地点收集的。总体而言，收集到的按蚊中有 20% 在白天活跃，旱季白天的叮咬量增加。 3131 个样本在形态学上被鉴定为 14 个不同物种，并且还通过 DNA 扩增子测序鉴定了一个子集，从而可以确定 29 个按蚊物种。对杀虫剂突变（ace-1、kdr 和 rdl 基因）的研究表明，个体携带与对所有不同类别杀虫剂的反应相关的突变。蚊子种类和杀虫剂抗药性之间似乎也存在非随机关联，其中按蚊表现出几乎固定的突变。疟原虫的分子筛选。存在表明，收集到的按蚊有 3.6% 呈阳性，其中大多数是间日疟原虫，其次是恶性疟原虫。这些结果强调了柬埔寨残留人类疟疾传播的一些关键机制，并说明了不同收集方法、地点和季节的重要性，以避免偏差并更好地描述东南亚按蚊生态特征。
In the Greater Mekong Subregion, malaria cases have significantly decreased but little is known about the vectors or mechanisms responsible for residual malaria transmission. We analysed a total of 3920 Anopheles mosquitoes collected during the rainy and dry seasons from four ecological settings in Cambodia (villages, forested areas near villages, rubber tree plantations and forest sites). Using odor-baited traps, 81% of the total samples across all sites were collected in cow baited traps, although 67% of the samples attracted by human baited traps were collected in forest sites. Overall, 20% of collected Anopheles were active during the day, with increased day biting during the dry season. 3131 samples were identified morphologically as 14 different species, and a subset was also identified by DNA amplicon sequencing allowing determination of 29 Anopheles species. The investigation of well characterized insecticide mutations (ace-1, kdr, and rdl genes) indicated that individuals carried mutations associated with response to all the different classes of insecticides. There also appeared to be a non-random association between mosquito species and insecticide resistance with Anopheles peditaeniatus exhibiting nearly fixed mutations. Molecular screening for Plasmodium sp. presence indicated that 3.6% of collected Anopheles were positive, most for P. vivax followed by P. falciparum. These results highlight some of the key mechanisms driving residual human malaria transmission in Cambodia, and illustrate the importance of diverse collection methods, sites and seasons to avoid bias and better characterize Anopheles mosquito ecology in Southeast Asia.
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DOI：
10.4269/ajtmh.1999.60.421
发表时间：
1999-03-01
影响因子：
3.3
作者：
Amerasinghe, PH;Amerasinghe, FP;Wirtz, RA
通讯作者：
Wirtz, RA
DOI：
10.1016/s0140-6736(15)00417-1
发表时间：
2016-04-23
期刊：
Lancet (London, England)
影响因子：
--
作者：
Hemingway J;Ranson H;Magill A;Kolaczinski J;Fornadel C;Gimnig J;Coetzee M;Simard F;Roch DK;Hinzoumbe CK;Pickett J;Schellenberg D;Gething P;Hoppé M;Hamon N
通讯作者：
Hamon N
DOI：
10.2987/8756-971x(2005)21
发表时间：
2005-09-01
影响因子：
1
作者：
Grieco, JP;Achee, NL;Andre, RG
通讯作者：
Andre, RG
影响因子：
3
作者：
Cuong Do Manh;Beebe, Nigel W.;Cooper, Robert D.
通讯作者：
Cooper, Robert D.
影响因子：
2.1
作者：
Chareonviriyaphap, T;Prabaripai, A;Aum-Aung, B
通讯作者：
Aum-Aung, B