Localization of propionibacterium acnes in granulomas supports a possible etiologic link between sarcoidosis and the bacterium.
Localization of propionibacterium acnes in granulomas supports a possible etiologic link between sarcoidosis and the bacterium.
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DOI：
10.1038/modpathol.2012.80
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发表时间：
2012-09
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影响因子：
--
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中科院分区：
文献类型：
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结节病可能是由于遗传易感对象暴露于环境因素，可能是传染性因素造成的。分枝杆菌和丙酸杆菌是最常见的潜在病原体。然而，痤疮丙酸杆菌是唯一的微生物，通过细菌培养在结节样病变中发现。为了评估这种本土细菌的致病作用，我们使用免疫组织化学方法，用新型痤疮假单抗与细胞膜结合的脂磷壁酸(PAB抗体)和核糖体结合的触发因子蛋白(TIG抗体)反应，对结节和非结节组织中的细菌进行筛选。我们检测了196例结节病患者的福尔马林固定和石蜡包埋的肺和淋巴结标本，以及275例非结节病患者的相应对照样本。样本大多来自日本患者，的淋巴结样本来自德国患者。PAB抗体免疫组织化学显示20/27(74%)胸科手术肺标本、24/50(48%)经纤维支气管镜肺活检标本、71/81(88%)日本淋巴结标本和34/38(89%)德国淋巴结标本可见结节样肉芽肿内的小圆体。在45例结核标本和34例结节样反应标本中，无一例与非结节样肉芽肿发生PAb抗体反应。在非肉芽肿区，许多结节和部分非结节患者的肺泡巨噬细胞和淋巴结皮质旁巨噬细胞内可见PAB抗体检测到的小圆形小体。在50%的结节和15%的非结节淋巴结标本中发现的大球形抗酸小体Hamazaki-Wesenberg小体与PAB和TIG抗体均呈阳性反应。电子显微镜显示，这些Hamazaki-Wesenberg小体只有一个细菌结构，没有细胞壁，偶尔会从身体伸出。PAB抗体在结节样肉芽肿中检测到痤疮假单胞菌的高频率和高特异性，提示这种本土细菌可能是许多结节病患者肉芽肿形成的原因。
Sarcoidosis likely results from the exposure of a genetically susceptible subject to an environmental agent, possibly an infectious one. Mycobacterial and propionibacterial organisms are the most commonly implicated potential etiologic agents. Propionibacterium acnes is the only microorganism, however, found in sarcoid lesions by bacterial culture. To evaluate the pathogenic role of this indigenous bacterium, we screened for the bacterium in sarcoid and non-sarcoid tissues using immunohistochemical methods with novel P. acnes-specific monoclonal antibodies that react with cell-membrane-bound lipoteichoic acid (PAB antibody) and ribosome-bound trigger-factor protein (TIG antibody). We examined formalin-fixed and paraffin-embedded samples of lungs and lymph nodes from 196 patients with sarcoidosis, and corresponding control samples from 275 patients with non-sarcoidosis diseases. The samples were mostly from Japanese patients, with 64 lymph node samples from German patients. Immunohistochemistry with PAB antibody revealed small round bodies within sarcoid granulomas in 20/27 (74%) video-assisted thoracic surgery lung samples, 24/50 (48%) transbronchial lung biopsy samples, 71/81 (88%) Japanese lymph node samples, and 34/38 (89%) German lymph node samples. PAB antibody did not react with non-sarcoid granulomas in any of the 45 tuberculosis samples or the 34 samples with sarcoid reaction. In nongranulomatous areas, small round bodies detected by PAB antibody were found in alveolar macrophages of lungs and paracortical macrophages of lymph nodes from many sarcoid and some non-sarcoid patients. Large-spheroidal acid-fast bodies, Hamazaki–Wesenberg bodies, which were found in 50% of sarcoid and 15% of non-sarcoid lymph node samples, reacted with both PAB and TIG antibodies. Electron microscopy revealed that these Hamazaki–Wesenberg bodies had a single bacterial structure and lacked a cell wall with occasional protrusions from the body. The high frequency and specificity of P. acnes, detected by PAB antibody within sarcoid granulomas, indicates that this indigenous bacterium might be the cause of granuloma formation in many sarcoid patients.
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影响因子：
3.3
作者：
Drake, Wonder Puryear;Newman, Lee S.
通讯作者：
Newman, Lee S.
影响因子：
24.3
作者：
Gupta, D.;Agarwal, R.;Jindal, S. K.
通讯作者：
Jindal, S. K.
影响因子：
3.1
作者：
Drake, Wonder P.;Dhason, Mary S.;Kalams, Spyros A.
通讯作者：
Kalams, Spyros A.
DOI：
10.1111/j.1432-1033.1983.tb07495.x
发表时间：
1983-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FISCHER, W;KOCH, HU;HAAS, R
通讯作者：
HAAS, R
DOI：
10.1007/bf02889023
发表时间：
1980-01-01
期刊：
VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY
影响因子：
--
作者：
CARR, I
通讯作者：
CARR, I