Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit.
Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit.
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乙烯信号转导元件参与非更年期枇杷果实冷害
DOI：
10.1093/jxb/erp302
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发表时间：
2010
影响因子：
6.9
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Wang P;Zhang B;Li X;Xu C;Yin X;Shan L;Ferguson I;Chen K
枇杷（Eriobotryajaponica Lindl.）属亚热带水果，部分品种如'洛阳青'（LYQ）易受冷害，而'白沙'（BS）等品种抗冷害。虽然枇杷是非跃变型，乙烯的调制成熟相关的收获后CI的影响。因此，乙烯信号在CI的发展中的作用进行了研究，在果实中的LYQ和BS品种。三个乙烯受体基因，一个CTR 1-like基因，和一个EIN 3-like基因的分离和鉴定成熟果实。所有这些基因在两个品种的果实内和果实之间都有差异表达。转录本在果实发育过程中下降（两个品种中的EjERS 1a和LYQ中的EjEIL 1），或在果实发育的中期下降（两个品种中的EjETR 1，EjERS 1b和EjCTR 1和BS中的EjEIL 1）。主要的品种差异是在水平，而不是在收获后储存过程中的表达模式。EjETR 1，EjCTR 1和EjEIL 1基因表现出增加的表达响应低温，这是特别值得注意的EjETR 1和EjEIL 1在CI发展过程中LYQ果实。这些基因对乙烯处理、1-甲基环丙烯（1-MCP）和低温处理也有不同的反应，证实了乙烯在枇杷果实CI调节中的作用。
Loquat (Eriobotrya japonica Lindl.) is a subtropical fruit, with some cultivars such as ‘Luoyangqing’ (LYQ) susceptible to chilling injury (CI), while others such as ‘Baisha’ (BS) are resistant. Although loquats are non-climacteric, modulation of ethylene has an effect on ripening-related post-harvest CI. Therefore the role of ethylene signalling in the development of CI was investigated in fruit of both the LYQ and BS cultivars. Three ethylene receptor genes, one CTR1-like gene, and one EIN3-like gene were isolated and characterized in ripening fruit. All of these genes were expressed differentially within and between fruit of the two cultivars. Transcripts either declined over fruit development (EjERS1a in both cultivars and EjEIL1 in LYQ) or showed an increase in the middle stages of fruit development before declining (EjETR1, EjERS1b, and EjCTR1 in both cultivars and EjEIL1 in BS). The main cultivar differences were in levels rather than in patterns of expression during post-harvest storage. EjETR1, EjCTR1, and EjEIL1 genes showed increased expression in response to low temperature and this was particularly notable for EjETR1, and EjEIL1 during CI development in LYQ fruit. The genes were also differentially responsive to ethylene treatment, 1-methycyclopropene (1-MCP) and low temperature conditioning, confirming a role for ethylene in regulation of CI in loquat fruit.
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影响因子：
7
作者：
Begheldo, Maura;Manganaris, George A.;Tonutti, Pietro
通讯作者：
Tonutti, Pietro
影响因子：
6.9
作者：
Iordachescu, M;Verlinden, S
通讯作者：
Verlinden, S
影响因子：
7
作者：
Dal Cin, Valeriano;Rizzini, Fabio Massimo;Tonutti, Pietro
通讯作者：
Tonutti, Pietro
影响因子：
7.4
作者：
Leclercq, J;Adams-Phillips, LC;Bouzayen, M
通讯作者：
Bouzayen, M
影响因子：
7
作者：
Girardi, CL;Corrent, AR;Rombaldi, CV
通讯作者：
Rombaldi, CV