Mystery solved: the identification of the two missing Romanov children using DNA analysis.

Mystery solved: the identification of the two missing Romanov children using DNA analysis.
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DOI:
10.1371/journal.pone.0004838
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发表时间:
2009
期刊:
影响因子:
3.7
通讯作者:
Finelli LN
Finelli LN
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Coble MD;Loreille OM;Wadhams MJ;Edson SM;Maynard K;Meyer CE;Niederstätter H;Berger C;Berger B;Falsetti AB;Gill P;Parson W;Finelli LN

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在20世纪的大部分时间里,最大的谜团之一是罗曼诺夫家族的命运,这是俄罗斯最后一个君主制国家。沙皇尼古拉斯二世退位后,他和他的妻子亚历山德拉以及他们的五个孩子最终被流放到叶卡捷琳堡市。这家人和他们的四名忠诚的工作人员被乌拉尔苏维埃成员俘虏。根据历史记载,1918年7月17日凌晨,全家及其四名忠诚的工作人员被行刑队处决。在一个废弃的矿井中处理遗体的尝试失败后,遗体被运送到距离矿井只有几公里的一片开阔的田野。这群人中的九名成员被埋葬在一个集体坟墓中,而两名儿童被埋葬在另一个坟墓中。1991年,官方发现了更大的乱葬坑,随后进行了DNA测试,以确认沙皇、沙皇和他们三个女儿的身份--人们一直怀疑这些遗骸实际上是罗曼诺夫家族的遗骸。2007年夏天,一群业余考古学家在距离较大的坟墓约70米的第二个坟墓中发现了一批遗骸。我们报告了2007年发现的遗骸的法医DNA检测,使用线粒体DNA(MtDNA)、常染色体STR和Y-STR测试。结合对1991年坟墓材料的额外DNA测试,我们有几乎无可辩驳的证据表明,从2007年坟墓中找到的两个人是罗曼诺夫家族失踪的两个孩子:察雷维奇·阿列克谢和他的一个姐妹。
One of the greatest mysteries for most of the twentieth century was the fate of the Romanov family, the last Russian monarchy. Following the abdication of Tsar Nicholas II, he and his wife, Alexandra, and their five children were eventually exiled to the city of Yekaterinburg. The family, along with four loyal members of their staff, was held captive by members of the Ural Soviet. According to historical reports, in the early morning hours of July 17, 1918 the entire family along with four loyal members of their staff was executed by a firing squad. After a failed attempt to dispose of the remains in an abandoned mine shaft, the bodies were transported to an open field only a few kilometers from the mine shaft. Nine members of the group were buried in one mass grave while two of the children were buried in a separate grave. With the official discovery of the larger mass grave in 1991, and subsequent DNA testing to confirm the identities of the Tsar, the Tsarina, and three of their daughters – doubt persisted that these remains were in fact those of the Romanov family. In the summer of 2007, a group of amateur archeologists discovered a collection of remains from the second grave approximately 70 meters from the larger grave. We report forensic DNA testing on the remains discovered in 2007 using mitochondrial DNA (mtDNA), autosomal STR, and Y- STR testing. Combined with additional DNA testing of material from the 1991 grave, we have virtually irrefutable evidence that the two individuals recovered from the 2007 grave are the two missing children of the Romanov family: the Tsarevich Alexei and one of his sisters.
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