Neutralizing antibody to interleukin 4 induces systemic protection and T helper type 1-associated immunity in murine candidiasis.
Neutralizing antibody to interleukin 4 induces systemic protection and T helper type 1-associated immunity in murine candidiasis.
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对白介素4的中和抗体诱导系统保护和T辅助型1型与鼠念珠菌病的免疫力。
DOI：
10.1084/jem.176.1.19
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发表时间：
1992-07-01
影响因子：
15.3
通讯作者：
BISTONI, F
中科院分区：
文献类型：
--
作者：
ROMANI, L;MENCACCI, A;GROHMANN, U;MOCCI, S;MOSCI, P;PUCCETTI, P;BISTONI, F
将白细胞介素4（IL-4）特异性单克隆抗体（mAb）给予全身感染酵母白色念珠菌的小鼠，并监测动物的死亡率、迟发型超敏反应的发生、不同同种型抗体的产生、IL-2、IL-4、IL-6和干扰素γ的释放通过脾CD 4+淋巴细胞在体外测定IL-4和IFN-γ的水平，以及这些细胞中IL-4和IFN-γ mRNA的水平。在几个独立的实验中，通过每周三次注射mAb中和IL-4，导致81%的总治愈率，而对照组为0%。治愈与有效清除感染器官中的酵母菌和疾病消退的组织学证据、检测到强的1型辅助性T细胞（Th 1）反应以及建立持久的保护性免疫相关。感染后不久，由于第一次或第二次注射mAb，CD 4+细胞中IL-4 mRNA减少，同时IFN-γ特异性转录物水平增加。因此，我们的数据表明，IL-4的Th 2细胞的生产可能会限制Th 1相关的保护性免疫小鼠念珠菌病。
An interleukin 4 (IL-4)-specific monoclonal antibody (mAb) was administered to mice infected systemically with the yeast Candida albicans, and the animals were monitored for mortality, development of delayed-type hypersensitivity, production of antibodies of different isotypes, release of IL-2, IL-4, IL-6, and interferon gamma (IFN-gamma) in vitro by splenic CD4+ lymphocytes, and levels of IL-4 and IFN-gamma mRNA in these cells. Neutralization of IL-4 by three weekly injections of mAb in several independent experiments resulted in an overall cure rate of 81% versus 0% of controls. Cure was associated with efficient clearance of the yeast from infected organs and histologic evidence of disease resolution, detection of strong T helper type 1 (Th1) responses, and establishment of long-lasting protective immunity. Soon after infection, and as a result of the first or second injection of mAb, there was a decrease in IL-4 mRNA in CD4+ cells, which was accompanied by an increase in the levels of IFN-gamma-specific transcripts. Our data thus indicate that the production of IL-4 by Th2 cells may limit Th1-associated protective immunity in murine candidiasis.
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影响因子：
3.1
作者：
CENCI, E;ROMANI, L;BISTONI, F
通讯作者：
BISTONI, F
影响因子：
64.8
作者：
OHARA, J;PAUL, WE
通讯作者：
PAUL, WE
影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N
影响因子：
3.1
作者：
BISTONI, F;VECCHIARELLI, A;CASSONE, A
通讯作者：
CASSONE, A
影响因子：
6.4
作者：
DOMER, JE
通讯作者：
DOMER, JE