Comparative genomics of white and opaque cell states supports an epigenetic mechanism of phenotypic switching in Candida albicans.
Comparative genomics of white and opaque cell states supports an epigenetic mechanism of phenotypic switching in Candida albicans.
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DOI：
10.1093/g3journal/jkab001
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发表时间：
2021-02-09
期刊：
影响因子：
--
通讯作者：
Ene IV
中科院分区：
文献类型：
--
作者：
Beekman CN;Cuomo CA;Bennett RJ;Ene IV
几个念珠菌属物种可以经历从“白色”状态到交配熟练的“不透明”状态的可遗传和可逆的转变。这种能力依赖于高度互连的转录网络，该网络控制多代细胞类型特异性基因表达程序。白色念珠菌是最主要的致病性念珠菌属物种，为白色-不透明转变提供了一个充分研究的范例。在该物种中，至少8个转录调节因子的网络控制具有不同形态、转录谱和生理特性的白色和不透明状态之间的平衡。鉴于可逆性和高频率的白色不透明转换，人们普遍认为这种转换是由独立于DNA序列中任何变化发生的表观遗传机制控制的。然而，白色和不透明细胞之间的直接基因组比较尚未进行。在此，我们提出了一个全基因组比较分析的C。白色念珠菌白色且不透明的细胞。这项分析揭示了细胞状态之间罕见的遗传变化，其中没有一个与白色不透明转换有关。这一结果与控制C.白念珠菌，并提供了直接的证据，对遗传变异的作用，介导开关。
Several Candida species can undergo a heritable and reversible transition from a ‘white’ state to a mating proficient ‘opaque’ state. This ability relies on highly interconnected transcriptional networks that control cell-type-specific gene expression programs over multiple generations. Candida albicans, the most prominent pathogenic Candida species, provides a well-studied paradigm for the white-opaque transition. In this species, a network of at least eight transcriptional regulators controls the balance between white and opaque states that have distinct morphologies, transcriptional profiles, and physiological properties. Given the reversible nature and the high frequency of white-opaque transitions, it is widely assumed that this switch is governed by epigenetic mechanisms that occur independently of any changes in DNA sequence. However, a direct genomic comparison between white and opaque cells has yet to be performed. Here, we present a whole-genome comparative analysis of C. albicans white and opaque cells. This analysis revealed rare genetic changes between cell states, none of which are linked to white-opaque switching. This result is consistent with epigenetic mechanisms controlling cell state differentiation in C. albicans and provides direct evidence against a role for genetic variation in mediating the switch.
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压力改变白色念珠菌中杂合性丧失的速率和类型。
影响因子：
6.4
作者：
Forche A;Abbey D;Pisithkul T;Weinzierl MA;Ringstrom T;Bruck D;Petersen K;Berman J
通讯作者：
Berman J
影响因子：
7
作者：
Brouard, Jean-Simon;Schenkel, Flavio;Bissonnette, Nathalie
通讯作者：
Bissonnette, Nathalie
DOI：
10.1084/jem.20101438
发表时间：
2010-10-25
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Christophersen NS;Helin K
通讯作者：
Helin K
影响因子：
3.3
作者：
Forche, Anja;Magee, P. T.;May, Georgiana
通讯作者：
May, Georgiana
影响因子：
3
作者：
Andaluz, Encarnacion;Gomez-Raja, Jonathan;Larriba, German
通讯作者：
Larriba, German