Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during In vitro maturation of reticulocytes.
Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during In vitro maturation of reticulocytes.
复制标题
在网织红细胞体外成熟过程中，外泌体内释放加速腐烂因子（CD55）和反应性裂解膜抑制剂（CD59）。
作者：
H. Rabesandratana;J. Toutant;H. Reggio;M. Vidal
外来体是网织红细胞在其成熟为红细胞期间释放的膜囊泡。它们具有清除功能，因为它们富集了一些已知在成熟过程中从细胞表面减少或消失的蛋白质，例如乙酰胆碱酯酶（AChE）和转铁蛋白受体（TfR）。为了更好地理解导致外泌体中蛋白质分选的分子事件，我们通过涉及珠偶联和流式细胞术免疫检测的技术分析了外泌体表面上糖基磷脂酰肌醇（GPI）锚定蛋白的表达。在从正常和阵发性睡眠性血红蛋白尿症（PNH）网织红细胞获得的外泌体表面上存在AChE、衰变加速因子（decay-accelerating factor，DIF）、反应性溶解的膜抑制剂（membrane inhibitor of reactive lysis，MIRL）和淋巴细胞功能相关抗原3（lymphocyte function-associated antigen 3，LFA-3），这表明（1）GPI锚在外泌体形成期间被有效分选，（2）外泌体释放可以解释PNH患者网织红细胞和红细胞之间GPI蛋白表达的差异，（3）外泌体可能具有与控制膜攻击复合物形成相关的另一种生理功能。
Exosomes are membrane vesicles released by reticulocytes during their maturation into erythrocytes. They have a clearing function because of their enrichment with some proteins known to decrease or disappear from the cell surface during maturation, eg, acetylcholinesterase (AChE) and transferrin receptor (TfR), respectively. To better understand the molecular events leading to protein sorting in exosomes, we analyzed the expression of glycosylphosphatidylinositol (GPI)-anchored proteins on the exosome surface through a technique involving bead coupling and flow cytometry immunodetection. The presence of AChE, decay-accelerating factor (DAF), membrane inhibitor of reactive lysis (MIRL), and lymphocyte function-associated antigen 3 (LFA-3) on the surface of exosomes obtained from normal and paroxysmal nocturnal hemoglobinuria (PNH) reticulocytes, suggests that (1) the GPI anchor is efficiently sorted during exosome formation, (2) exosome release could account for the observed discrepancy in GPI-protein expression between reticulocytes and erythrocytes from PNH patients, and (3) exosomes could have another physiologic function related to controlling membrane attack complex formation.
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人红细胞乙酰胆碱酯酶 C 末端糖脂膜结合域中胺成分的鉴定。
影响因子：
2.9
作者：
Haas,R;Brandt,PT;Knight,J;Rosenberry,TL
通讯作者：
Rosenberry,TL
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DOI：
10.1111/j.1432-1033.1990.tb15274.x
发表时间：
1990
期刊：
European journal of biochemistry
影响因子：
--
作者：
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通讯作者：
Rosenberry,TL
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影响因子：
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作者：
Yomtovian,R;Prince,GM;Medof,ME
通讯作者：
Medof,ME
影响因子：
56.9
作者：
KOOYMAN, DL;BYRNE, GW;LOGAN, JS
通讯作者：
LOGAN, JS