Effects of LPS on the Secretion of Gonadotrophin Hormones and Expression of Genes in the Hypothalamus-Pituitary-Ovary (HPG) Axis in Laying Yangzhou Geese.
Effects of LPS on the Secretion of Gonadotrophin Hormones and Expression of Genes in the Hypothalamus-Pituitary-Ovary (HPG) Axis in Laying Yangzhou Geese.
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LPS对扬州蛋蛋促性腺激素分泌及下丘脑-垂体-卵巢(HPG)轴基因表达的影响
DOI：
10.3390/ani10122259
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发表时间：
2020-11-30
期刊：
影响因子：
--
通讯作者：
Shi Z
中科院分区：
文献类型：
--
作者：
Ying S;Qin J;Dai Z;An H;Zhu H;Chen R;Yang X;Wu W;Shi Z
脂多糖是一种来自大肠杆菌的内毒素，已被证明对哺乳动物的卵泡发育和类固醇合成、垂体和下丘脑生殖激素的分泌具有损害作用。然而，内毒素对鸟类生殖轴的影响仍然不清楚。据报道，由于病原细菌在含有病原的水体上的特殊交配行为，导致产蛋率下降，给养鹅生产造成经济损失。本研究表明，内毒素感染后，扬州鹅血浆中垂体促性腺激素水平和生殖轴基因表达发生了改变。值得注意的是，我们首次证明了内毒素处理后，下丘脑中两个重要的生殖基因促性腺激素释放激素(GnRH)和促性腺激素抑制激素(GnIH)的表达都发生了变化。我们的结果可以解释细菌感染后鹅产蛋率下降的原因，也为研究内毒素引起的繁殖障碍和免疫攻击提供了新的见解。摘要高放养密度鹅夏季产蛋性能下降与革兰氏阴性菌产生的脂多糖有关。注射脂多糖延缓了产蛋率的提高，改变了卵泡的分级形态。虽然有证据表明内毒素对鹅繁殖有抑制作用，但内毒素对下丘脑-垂体-卵巢(HPG)轴的时程影响仍不清楚。本试验研究了鹅静脉注射脂多糖后0、6、12、24、36h HPG轴基因表达和血浆促性腺激素水平的变化。结果表明，内毒素可促进和抑制下丘脑促性腺激素抑制激素(GnIH)和促性腺激素释放激素(GnRH)mRNA的表达，对其受体GnIHR和GnRHR的mRNA表达也有相似的影响。脂多糖处理后12h，卵泡FSHβ基因表达一过性增加，而对β基因表达无明显影响。无论促卵泡激素β和促黄体生成素β的表达如何，血浆促卵泡刺激素和黄体生成素浓度在内毒素处理后24~36h均显著升高。在卵巢中，StAR和Cyp11a1主要在分级卵泡的颗粒层(GL)表达，而CYP17A1和CYP19A1主要在白色卵泡(WF)和淡黄色卵泡(YF)中表达，在卵膜层(TL)中表达较少。内毒素处理后，GLS中的Cyp11a1、WFS和TL中的CYP17A1以及WFS、YFS和TL中的CYP19A1的mRNA水平均显著降低。但在12h时，脂多糖处理可使STAR表达短暂上调，提示脂多糖暴露可能损害HPG轴，干扰卵巢类固醇激素的合成。我们的研究对脂多糖引起的鸟类生殖功能障碍和免疫挑战提供了新的见解。
Simple Summary Lipopolysaccharide (LPS), an endotoxin from E. coli, has been proven to impair follicle development and steroidogenesis, secretion of pituitary and hypothalamus reproductive hormones in mammals. However, the effects of LPS on the avian reproductive axis remain elusive. Pathogenic bacterial infection due to the particular mating behavior on the water containing pathogens was reported to decrease the laying rate and cause economic loss in goose production. In this study, we showed that LPS infection disturbed the plasma pituitary gonadotrophin hormone concentrations and the gene expression of the reproductive axis in Yangzhou geese. Notably, for the first time we proved that both the expression of gonadotrophin-releasing hormone (GnRH) and gonadotropin-inhibiting hormone (GnIH), two important reproductive genes from the hypothalamus, were altered after LPS treatment in birds. Our results can explain the decreased laying rate in goose after bacterial infection, and also provide new insights into reproductive dysfunction caused by LPS and the immune challenge in birds. Abstract Lipopolysaccharide (LPS) from gram-negative bacteria was found to be involved in the decrease in laying performance in goose flocks with high stocking density during summer months. LPS injection delayed the increase in the laying rate and altered hierarchical follicle morphology. While there is evidence that LPS exerts suppressive effects on goose reproduction, the time course effects of LPS on the hypothalamus-pituitary-ovary (HPG) axis remain elusive. In this study, we investigated the expression of genes in the HPG axis and the plasma gonadotrophin hormone concentrations in breeding geese at 0, 6, 12, 24, and 36 h after intravenous injection with LPS. The results showed that LPS treatment enhanced and suppressed expression of hypothalamic gonadotropin-inhibiting hormone (GnIH) and gonadotrophin-releasing hormone (GnRH) mRNA, respectively, and similar effects were observed on the mRNA expression of their receptors, GnIHR and GnRHR, in the pituitary. LPS treatment transiently increased follicle FSHβ mRNA expression at 12 h and exerted no significant effect on LHβ mRNA expression in the pituitary. Regardless of the expression of FSHβ and LHβ, plasma follicle stimulating hormone (FSH) and luteinizing hormone (LH) concentrations were significantly increased during 24–36 h after LPS treatment. In the ovary, StAR and Cyp11a1 were mainly expressed in the granulosa layer (GL) of hierarchical follicles, while Cyp17a1 and Cyp19a1 were mainly expressed in white follicles (WFs) and yellowish follicles (YFs), and to a lesser extent in the theca layer (TL). After LPS treatment, the mRNA levels of Cyp11a1 in the GLs, Cyp17a1 in the WFs and TL, and Cyp19a1 in the WFs, YFs, and TL were significantly decreased. However, LPS treatment transiently upregulated StAR expression at 12 h. These results indicate that the exposure of laying geese to LPS may impair the HPG axis and disturb ovarian steroidogenesis. Our research provides new insights into reproductive dysfunction caused by LPS and the immune challenge in birds.
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