Vaccination of calves with Mycobacterium bovis Bacillus Calmette-Guerin reduces the frequency and severity of lesions of bovine tuberculosis under a natural transmission setting in Ethiopia.
Vaccination of calves with Mycobacterium bovis Bacillus Calmette-Guerin reduces the frequency and severity of lesions of bovine tuberculosis under a natural transmission setting in Ethiopia.
复制标题
DOI：
10.1111/tbed.12618
复制
发表时间：
2018-03
影响因子：
4.3
通讯作者：
Vordermeier HM
中科院分区：
文献类型：
--
作者：
Ameni G;Tafess K;Zewde A;Eguale T;Tilahun M;Hailu T;Sirak A;Salguero FJ;Berg S;Aseffa A;Hewinson RG;Vordermeier HM
牛结核病 (bTB) 在埃塞俄比亚城市“奶棚”的集约化奶牛场中非常流行，疫苗接种可能是一种经济有效的疾病控制策略。在本研究中，在自然传播环境下的荷斯坦-弗里斯兰小牛中评估了卡介苗 (BCG) 预防 bTB 的功效。 23 头 2 周大的小牛皮下接种了 BCG 丹麦 SSI 菌株 1331，并为 26 头匹配的小牛注射了安慰剂。六周后，小牛被引入一群感染牛支原体的动物（反应器）中，并与它们接触一年。进行体外和体内免疫学测试，以评估疫苗接种后和暴露期间的免疫反应。通过结核菌素皮试和接种牛犊的 IFN-γ 反应证实了疫苗的成功接种。 IFN-γ 对早期分泌抗原靶标 6 和培养物滤过蛋白 10（分别为 ESAT6 和 CFP10）的反应动力学以及暴露后结核菌素皮试反应表明，动物在与受感染牛群接触后很早就被感染，因为在初次暴露后 2 至 4 个月内可测量到感染的免疫学迹象。通过比较接种小牛和对照小牛之间的总体和微观病理学以及细菌学负担来确定保护作用。与对照犊牛相比，BCG 疫苗接种使接种牛犊中具有可见病理的组织比例减少了 49% (p < .001)，其中头部、胸部、腹部淋巴结和肺部病变组织的比例分别减少了 56%、43%、72% 和 38%（p 值 0.029-.0001）。此外，与未接种疫苗的小牛相比，接种疫苗的小牛的病变在肉眼和显微镜下均不那么严重 (p < .05)。由于接种疫苗的动物没有大体病理学、牛分枝杆菌培养阳性和组织病理学结果，bTB 的总体发病率分别降低了 23%、28% 和 33%。总之，卡介苗疫苗接种可显着降低 bTB 病理的频率和严重程度，这可能会减少该疾病的进一步传播。
Bovine tuberculosis (bTB) is highly prevalent in intensive dairy farms of the urban “milk‐sheds” in Ethiopia, and vaccination could be a cost‐effective disease control strategy. In the present study, the efficacy of Bacillus Calmette–Guerin (BCG) to protect against bTB was assessed in Holstein–Friesian calves in a natural transmission setting. Twenty‐three 2‐week‐old calves were subcutaneously vaccinated with BCG Danish SSI strain 1331, and matched 26 calves were injected with placebo. Six weeks later, calves were introduced into a herd of M. bovis‐infected animals (reactors) and kept in contact with them for 1 year. In vitro and in vivo immunological tests were performed to assess immune responses post‐vaccination and during exposure. Successful vaccine uptake was confirmed by tuberculin skin test and IFN‐γ responses in vaccinated calves. The kinetics of IFN‐γ responses to early secretory antigen target 6 and culture filtrate protein 10 (ESAT6 and CFP10, respectively) and tuberculin skin test responses post‐exposure suggested that the animals were infected early after being placed in contact with the infected herd as immunological signs of infection were measurable between 2 and 4 months post‐initial exposure. Protection was determined by comparing gross and microscopic pathology and bacteriological burden between vaccinated and control calves. BCG vaccination reduced the proportions of tissues with visible pathology in vaccinates compared to control calves by 49% (p < .001) with 56%, 43%, 72%, and 38% reductions in the proportion of lesioned tisues in head, thoracic, abdominal lymph nodes, and lungs, respectively (p‐values .029–.0001). In addition, the lesions were less severe grossly and microscopically in vaccinated calves than in non‐vaccinated calves (p < .05). The reduction in the overall incidence rates of bTB was 23%, 28%, and 33% on the basis of the absence of gross pathology, M. bovis culture positivity, and histopathology, respectively, in vaccinated animals. In conclusion, BCG vaccination reduced the frequency and severity of the pathology of bTB significantly, which is likely to reduce onwards transmission of the disease.
登录
查看更多内容
影响因子：
2.2
作者：
Tschopp, R.;Schelling, E.;Zinsstag, J.
通讯作者：
Zinsstag, J.
影响因子：
3.7
作者：
Firdessa R;Tschopp R;Wubete A;Sombo M;Hailu E;Erenso G;Kiros T;Yamuah L;Vordermeier M;Hewinson RG;Young D;Gordon SV;Sahile M;Aseffa A;Berg S
通讯作者：
Berg S
影响因子：
--
作者：
Ameni, Gobena;Aseffa, Abraham;Vordermeier, Martin
通讯作者：
Vordermeier, Martin
影响因子：
11.8
作者：
Cosivi, O;Grange, JM;Meslin, FX
通讯作者：
Meslin, FX
影响因子：
3.2
作者：
Hewinson, RG;Vordermeier, HM;Buddle, BM
通讯作者：
Buddle, BM