High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research.
High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research.
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DOI：
10.1038/s41598-023-43233-4
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发表时间：
2023-09-27
影响因子：
4.6
通讯作者：
Trimmer, James S.
中科院分区：
文献类型：
--
作者：
Mitchell, Keith G.;Gong, Belvin;Hunter, Samuel S.;Burkart-Waco, Diana;Gavira-O'Neill, Clara E.;Templeton, Kayla M.;Goethel, Madeline E.;Bzymek, Malgorzata;MacNiven, Leah M.;Murray, Karl D.;Settles, Matthew L.;Froenicke, Lutz;Trimmer, James S.
神经科学单克隆抗体测序计划（NeuroMabSeq）是一项共同努力，旨在确定并公开提供对神经科学研究有价值的杂交瘤衍生单克隆抗体（mAb）序列。超过30年的研究和开发工作，包括那些在加州大学戴维斯分校/NIH NeuroMab设施已经导致产生了大量的小鼠单克隆抗体验证神经科学研究。为了提高传播和增加这一宝贵资源的效用，我们应用高通量DNA测序方法来确定来源杂交瘤细胞的免疫球蛋白重链和轻链可变结构域序列。所得到的序列集作为可搜索的DNA序列数据库（neuromabseq.ucdavis.edu）公开提供，用于下游应用中的共享、分析和使用。通过使用这些序列开发重组mAb，我们增强了现有mAb收集的实用性、透明度和再现性。这使得它们能够随后工程化为具有独特效用的替代形式，包括多重标记中的替代检测模式，以及作为小型化单链可变片段或scFv。NeuroMabSeq网站和数据库以及相应的重组抗体集合一起作为小鼠mAb重链和轻链可变结构域序列的公共DNA序列库，并作为用于增强这种经验证的mAb的有价值集合的传播和效用的开放资源。
The Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq) is a concerted effort to determine and make publicly available hybridoma-derived sequences of monoclonal antibodies (mAbs) valuable to neuroscience research. Over 30 years of research and development efforts including those at the UC Davis/NIH NeuroMab Facility have resulted in the generation of a large collection of mouse mAbs validated for neuroscience research. To enhance dissemination and increase the utility of this valuable resource, we applied a high-throughput DNA sequencing approach to determine immunoglobulin heavy and light chain variable domain sequences from source hybridoma cells. The resultant set of sequences was made publicly available as a searchable DNA sequence database (neuromabseq.ucdavis.edu) for sharing, analysis and use in downstream applications. We enhanced the utility, transparency, and reproducibility of the existing mAb collection by using these sequences to develop recombinant mAbs. This enabled their subsequent engineering into alternate forms with distinct utility, including alternate modes of detection in multiplexed labeling, and as miniaturized single chain variable fragments or scFvs. The NeuroMabSeq website and database and the corresponding recombinant antibody collection together serve as a public DNA sequence repository of mouse mAb heavy and light chain variable domain sequences and as an open resource for enhancing dissemination and utility of this valuable collection of validated mAbs.
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影响因子：
14.9
作者：
Dunbar J;Krawczyk K;Leem J;Marks C;Nowak J;Regep C;Georges G;Kelm S;Popovic B;Deane CM
通讯作者：
Deane CM
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.8
作者：
Dunbar, James;Deane, Charlotte M.
通讯作者：
Deane, Charlotte M.
影响因子：
2.5
作者：
Lipman, NS;Jackson, LR;Weis-Garcia, F
通讯作者：
Weis-Garcia, F
影响因子：
3.6
作者：
CARROLL, WL;MENDEL, E;LEVY, S
通讯作者：
LEVY, S