Transplantation of Nogo-66 receptor gene-silenced cells in a poly(D,L-lactic-co-glycolic acid) scaffold for the treatment of spinal cord injury.
Transplantation of Nogo-66 receptor gene-silenced cells in a poly(D,L-lactic-co-glycolic acid) scaffold for the treatment of spinal cord injury.
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DOI：
10.3969/j.issn.1673-5374.2013.08.001
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发表时间：
2013-03-15
影响因子：
6.1
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Wang D;Fan Y;Zhang J
神经突生长的抑制很大程度上是由 Nogo-66 受体介导的，会影响骨髓间充质干细胞移植后的神经再生。本发明的组织工程支架聚(D,L-丙交酯-乙醇酸)具有良好的组织相容性，能够促进再生神经纤维的生长。本研究使用小干扰RNA沉默骨髓间充质干细胞和雪旺细胞中Nogo-66受体基因的表达，随后将其用聚(D,L-丙交酯-乙醇酸)移植到大鼠脊髓病变区域。同时将仅给予支架治疗的大鼠作为对照组。苏木精-伊红染色和免疫组化结果显示，移植后4周，Nogo-66受体基因沉默细胞加聚(D,L-丙交酯-乙醇酸)支架治疗的大鼠与单纯支架治疗的大鼠相比，后肢运动功能良好，骨髓间充质干细胞和神经元样细胞的数量也较单纯支架治疗的大鼠有良好的后肢运动功能。 增加。移植后8周，辣根过氧化物酶示踪和透射电子显微镜显示大量无髓鞘和有髓神经纤维，以及脊髓半切损伤后完整的再生轴突髓鞘。这些实验结果表明，Nogo-66受体基因沉默的骨髓间充质干细胞和雪旺细胞加聚(D,L-丙交酯-乙醇酸)支架的移植可以显着增强脊髓神经元的轴突再生，改善脊髓损伤后大鼠的四肢运动功能。
Inhibition of neurite growth, which is in large part mediated by the Nogo-66 receptor, affects neural regeneration following bone marrow mesenchymal stem cell transplantation. The tissue engineering scaffold poly(D,L-lactide-co-glycolic acid) has good histocompatibility and can promote the growth of regenerating nerve fibers. The present study used small interfering RNA to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells and Schwann cells, which were subsequently transplanted with poly(D,L-lactide-co-glycolic acid) into the spinal cord lesion regions in rats. Simultaneously, rats treated with scaffold only were taken as the control group. Hematoxylin-eosin staining and immunohistochemistry revealed that at 4 weeks after transplantation, rats had good motor function of the hind limb after treatment with Nogo-66 receptor gene-silenced cells plus the poly(D,L-lactide-co-glycolic acid) scaffold compared with rats treated with scaffold only, and the number of bone marrow mesenchymal stem cells and neuron-like cells was also increased. At 8 weeks after transplantation, horseradish peroxidase tracing and transmission electron microscopy showed a large number of unmyelinated and myelinated nerve fibers, as well as intact regenerating axonal myelin sheath following spinal cord hemisection injury. These experimental findings indicate that transplantation of Nogo-66 receptor gene-silenced bone marrow mesenchymal stem cells and Schwann cells plus a poly(D,L-lactide-co-glycolic acid) scaffold can significantly enhance axonal regeneration of spinal cord neurons and improve motor function of the extremities in rats following spinal cord injury.
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影响因子：
2.5
作者：
Stenberg, Lena;Kanje, Martin;Dahlin, Lars B.
通讯作者：
Dahlin, Lars B.
影响因子：
10.8
作者：
Mittal, G.;Sahana, D. K.;Kumar, M. N. V. Ravi
通讯作者：
Kumar, M. N. V. Ravi
影响因子：
2.9
作者：
Ban, De-Xiang;Kong, Xiao-Hong;Guo, Shi-Fu
通讯作者：
Guo, Shi-Fu
DOI：
10.1073/pnas.052678899
发表时间：
2002-03-05
影响因子：
11.1
作者：
Teng, YD;Lavik, EB;Snyder, EY
通讯作者：
Snyder, EY
影响因子：
3.3
作者：
Parr, A. M.;Kulbatski, I.;Tator, C. H.
通讯作者：
Tator, C. H.