Beneficial Effects of Dietary Nitrite on a Model of Nonalcoholic Steatohepatitis Induced by High-Fat/High-Cholesterol Diets in SHRSP5/Dmcr Rats: A Preliminary Study.
Beneficial Effects of Dietary Nitrite on a Model of Nonalcoholic Steatohepatitis Induced by High-Fat/High-Cholesterol Diets in SHRSP5/Dmcr Rats: A Preliminary Study.
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DOI：
10.3390/ijms23062931
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发表时间：
2022-03-08
影响因子：
5.6
通讯作者：
Kobayashi J
中科院分区：
文献类型：
--
作者：
Sonoda K;Kono Y;Kitamori K;Ohtake K;Shiba S;Kasono K;Kobayashi J
非酒精性脂肪性肝炎（NASH）是一种慢性肝病，可导致肝硬化和肝细胞癌。肝脂毒性引起的内皮功能障碍是在肝脏和心血管系统中观察到的潜在NASH病理。在这里，我们评估了膳食亚硝酸盐对大鼠NASH模型的影响。采用高脂/高胆固醇饮食建立NASH模型，并在饮用水中添加亚硝酸盐或卡托普利（各100 mg/L），持续8周。通过肝脏和心脏组织的免疫组织化学分析来评估亚硝酸盐和卡托普利的作用。通过测量烟酰胺腺嘌呤二核苷酸磷酸氧化酶成分和肝脏炎症细胞积累的蛋白质水平，膳食亚硝酸盐通过减少氧化应激来抑制大鼠的肝纤维化。亚硝酸盐降低高血压NASH大鼠血压，抑制左心室增大。在卡托普利治疗的大鼠NASH模型中观察到类似的治疗效果，提示亚硝酸盐和卡托普利可能通过共同的信号通路改善NASH病理。综上所述，膳食亚硝酸盐可减轻大鼠合并心血管疾病的NASH发展，并为NASH治疗提供了一种替代策略。
Nonalcoholic steatohepatitis (NASH) is a chronic liver disease that leads to liver cirrhosis and hepatocellular carcinoma. Endothelial dysfunction caused by hepatic lipotoxicity is an underlying NASH pathology observed in the liver and the cardiovascular system. Here, we evaluated the effect of dietary nitrite on a rat NASH model. Stroke-prone, spontaneously hypertensive 5/Dmcr rats were fed a high-fat/high-cholesterol diet to develop the NASH model, with nitrite or captopril (100 mg/L, each) supplementation in drinking water for 8 weeks. The effects of nitrite and captopril were evaluated using immunohistochemical analyses of the liver and heart tissues. Dietary nitrite suppressed liver fibrosis in the rats by reducing oxidative stress, as measured using the protein levels of nicotinamide adenine dinucleotide phosphate oxidase components and inflammatory cell accumulation in the liver. Nitrite lowered the blood pressure in hypertensive NASH rats and suppressed left ventricular chamber enlargement. Similar therapeutic effects were observed in a captopril-treated rat NASH model, suggesting the possibility of a common signaling pathway through which nitrite and captopril improve NASH pathology. In conclusion, dietary nitrite attenuates the development of NASH with cardiovascular involvement in rats and provides an alternative NASH therapeutic strategy.
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影响因子：
3
作者：
Horai, Yasushi;Utsumi, Hiroyuki;Fukunari, Atsushi
通讯作者：
Fukunari, Atsushi
DOI：
10.1016/j.niox.2021.08.001
发表时间：
2021-10-01
期刊：
Nitric oxide : biology and chemistry
影响因子：
--
作者：
Kobayashi J
通讯作者：
Kobayashi J
影响因子：
10.8
作者：
Kupfahl, C;Pink, D;Regitz-Zagrosek, V
通讯作者：
Regitz-Zagrosek, V
影响因子：
16.1
作者：
Higashi T;Friedman SL;Hoshida Y
通讯作者：
Hoshida Y
影响因子：
4.3
作者：
Djekoun-Bensoltane, S.;Kammerer, M.;Thorin, C.
通讯作者：
Thorin, C.