Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula.
Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula.
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蒺藜苜蓿 PP2C 基因的全基因组鉴定及其响应干旱和冷胁迫的表达谱
DOI：
10.1038/s41598-018-29627-9
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发表时间：
2018-08-27
影响因子：
4.6
通讯作者：
Wang R
中科院分区：
文献类型：
--
作者：
Yang Q;Liu K;Niu X;Wang Q;Wan Y;Yang F;Li G;Wang Y;Wang R
2型C蛋白磷酸酶（Type 2c protein phosphatases, pp2c）是植物中最主要的一类蛋白磷酸酶，在植物的各种生理过程中起着重要作用。本研究共鉴定出94个MtPP2C基因，并根据外显子-内含子结构和保守基序组成将其系统发育分为13个亚科。共线性分析表明，片段重复事件在短尾曲霉MtPP2C基因家族的扩增中起着至关重要的作用。利用qRT-PCR分析不同非生物处理下MtPP2Cs的表达谱。结果表明，这些MtPP2Cs基因在干旱、寒冷和ABA胁迫条件下表现出不同的表达模式，并已鉴定出一些关键的MtPP2Cs基因。我们的研究全面概述了M. truncatula的PP2C基因家族，这将有助于进一步表征mtpp2c在植物干旱和冷胁迫响应中的功能。
Type 2 C protein phosphatases (PP2Cs) represent the major group of protein phosphatases in plants and play important roles in various plant processes. In this study, 94 MtPP2C genes were identified from Medicago truncatula and further phylogenetically classified into 13 subfamilies, as supported by exon-intron organization and conserved motif composition. Collinearity analysis indicated that segmental duplication events played a crucial role in the expansion of MtPP2C gene families in M. truncatula. Furthermore, the expression profiles of MtPP2Cs under different abiotic treatments were analyzed using qRT-PCR. Results showed that these MtPP2Cs genes displayed different expression patterns in response to drought, cold and ABA stress conditions and some of the key stress responsive MtPP2Cs genes have been identified. Our study presents a comprehensive overview of the PP2C gene family in M. truncatula, which will be useful for further functional characterization of MtPP2Cs in plant drought and cold stress responses.
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影响因子：
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通讯作者：
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