Intestinal replication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus.
Intestinal replication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus.
复制标题
DOI：
10.1016/0378-1135(90)90154-n
复制
发表时间：
1990-06
影响因子：
3.3
通讯作者：
van Deun K
中科院分区：
文献类型：
--
作者：
Cox E;Pensaert MB;Callebaut P;van Deun K
将猪呼吸道冠状病毒（PRCV）经静脉或直接接种到一周龄仔猪的胃肠道内腔中。两种接种途径均导致从尾部小肠分离出病毒。然而，仅在消化道接种≥ 103 TCID50时观察到病毒复制。复制仍然局限于少数身份不明的细胞位于或下面的上皮层绒毛或隐窝网站。病毒在粪便中排出数天，但呼吸道感染在同一猪中很少发生。本研究的结果表明，PRCV和传染性胃肠炎病毒之间的分子结构的微小变化导致宿主细胞嗜性的重要变化。
One-week-old piglets were inoculated with the porcine respiratory coronavirus (PRCV) either intravenously or directly into the lumen of the gastrointestinal tract. Both inoculation routes resulted in the isolation of virus from the caudal small intestine. Viral replication, however, was only observed upon inoculation into the digestive tract in quantities of ≥ 103 TCID50. Replication remained limited to a few unidentified cells located in or underneath the epithelial layer at villus- or crypt-sites. Virus was excreted in the faeces for several days but infection of the respiratory tract occured rarely in the same pigs. The results of this study indicate that small changes in molecular structure between PRCV and transmissible gastroenteritis virus have resulted in important changes in host cell tropism.
影响因子：
2.4
作者：
COX, E;HOOYBERGHS, J;PENSAERT, MB
通讯作者：
PENSAERT, MB
影响因子：
3.3
作者：
CALLEBAUT, P;PENSAERT, MB;HOOYBERGHS, J
通讯作者：
HOOYBERGHS, J
DOI：
10.1007/bf01253767
发表时间：
1970-01-01
期刊：
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG
影响因子：
--
作者：
PENSAERT, M;HAELTERMAN, EO;BURNSTEIN, T
通讯作者：
BURNSTEIN, T