Meta-analysis of studies using suppression subtractive hybridization and microarrays to investigate the effects of environmental stress on gene transcription in oysters.
Meta-analysis of studies using suppression subtractive hybridization and microarrays to investigate the effects of environmental stress on gene transcription in oysters.
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DOI：
10.1371/journal.pone.0118839
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Raftos DA
中科院分区：
文献类型：
--
作者：
Anderson K;Taylor DA;Thompson EL;Melwani AR;Nair SV;Raftos DA
许多微阵列和抑制性消减杂交（SSH）研究分析了环境胁迫对海洋物种基因转录的影响。然而，还没有统一的分析这些数据，以确定共同的压力反应途径。为了解决这一不足，我们对14项研究进行了荟萃分析，这些研究调查了不同环境压力对牡蛎基因表达的影响。测试的压力源包括化学污染，缺氧和感染，以及极端的温度，pH值和浊度。我们发现，超过400个基因的表达在一系列牡蛎物种暴露于环境胁迫后发生了显着变化。一个重复的模式是显而易见的，在这些转录反应，无论施加的压力类型。许多对环境压力作出反应的基因编码参与翻译和蛋白质加工（包括分子伴侣）、线粒体电子传递链、抗氧化活性和细胞骨架的蛋白质。根据这些发现，我们提出了一个共识模型的亚细胞应激反应的牡蛎。
Many microarray and suppression subtractive hybridization (SSH) studies have analyzed the effects of environmental stress on gene transcription in marine species. However, there have been no unifying analyses of these data to identify common stress response pathways. To address this shortfall, we conducted a meta-analysis of 14 studies that investigated the effects of different environmental stressors on gene expression in oysters. The stressors tested included chemical contamination, hypoxia and infection, as well as extremes of temperature, pH and turbidity. We found that the expression of over 400 genes in a range of oyster species changed significantly after exposure to environmental stress. A repeating pattern was evident in these transcriptional responses, regardless of the type of stress applied. Many of the genes that responded to environmental stress encoded proteins involved in translation and protein processing (including molecular chaperones), the mitochondrial electron transport chain, anti-oxidant activity and the cytoskeleton. In light of these findings, we put forward a consensus model of sub-cellular stress responses in oysters.
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