Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction.
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DOI：
10.1016/j.bioactmat.2023.10.034
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发表时间：
2024-03
影响因子：
18.9
通讯作者：
中科院分区：
文献类型：
--
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颅颌面(CMF)重建是一个具有挑战性的临床难题。它通常需要以自体移植或皮瓣手术的形式进行皮肤置换，这些手术根据皮下/真皮含量的不同而不同。不幸的是，这两种方法都受到疤痕形成、美容效果差、本土解剖和头发修复不充分、脱发、供区发病率和失败的可能性的困扰。因此，新的重建方法是必要的，而组织工程皮肤是一种令人兴奋的选择。在这项研究中，我们展示了使用术中生物打印(IOB)重建CMF全层皮肤缺损，这使得能够在外科环境下通过直接对免疫缺陷大鼠的多层皮肤进行生物打印来修复缺陷。使用一种新研制的患者来源的同种异体生物墨水，包括人类脂肪来源的细胞外基质(AdECM)和干细胞(ADSCs)，通过在三组不同的动物实验中精确地沉积真皮和真皮成分来重建皮肤丢失。AdECM即使在非常低的浓度下，如2%或更低，也被证明可以通过基于液滴的生物打印进行生物打印，并在体外和体内都显示出新的成脂能力。我们的研究结果表明，ADECM和ADSCs的联合应用促进了三个全层皮肤缺损的重建，在两周内完成了几乎完全的伤口闭合。更重要的是，在这两周的时间框架内，皮下脂肪生成和毛囊样结构的下行生长都实现了。我们的方法展示了使用人类衍生材料和IOB技术进行全层皮肤剥离的翻译潜力。颅颌面部重建是一个具有挑战性的临床难题通过精确沉积真皮和真皮成分来构建皮肤AdECM是可生物打印的，并具有新的成脂能力。AdECM和ADSCs的联合应用促进了皮肤的重建，实现了毛囊样结构的脂肪生成和下行生长
Craniomaxillofacial (CMF) reconstruction is a challenging clinical dilemma. It often necessitates skin replacement in the form of autologous graft or flap surgery, which differ from one another based on hypodermal/dermal content. Unfortunately, both approaches are plagued by scarring, poor cosmesis, inadequate restoration of native anatomy and hair, alopecia, donor site morbidity, and potential for failure. Therefore, new reconstructive approaches are warranted, and tissue engineered skin represents an exciting alternative. In this study, we demonstrated the reconstruction of CMF full-thickness skin defects using intraoperative bioprinting (IOB), which enabled the repair of defects via direct bioprinting of multiple layers of skin on immunodeficient rats in a surgical setting. Using a newly formulated patient-sourced allogenic bioink consisting of both human adipose-derived extracellular matrix (adECM) and stem cells (ADSCs), skin loss was reconstructed by precise deposition of the hypodermal and dermal components under three different sets of animal studies. adECM, even at a very low concentration such as 2 % or less, has shown to be bioprintable via droplet-based bioprinting and exhibited de novo adipogenic capabilities both in vitro and in vivo. Our findings demonstrate that the combinatorial delivery of adECM and ADSCs facilitated the reconstruction of three full-thickness skin defects, accomplishing near-complete wound closure within two weeks. More importantly, both hypodermal adipogenesis and downgrowth of hair follicle-like structures were achieved in this two-week time frame. Our approach illustrates the translational potential of using human-derived materials and IOB technologies for full-thickness skin loss. Craniomaxillofacial reconstruction is a challenging clinical dilemma Skin was built by precise deposition of hypodermal and dermal components AdECM was bioprintable and exhibited de novo adipogenic capabilities Combinatorial delivery of adECM and ADSCs facilitated the reconstruction of skin Adipogenesis and downgrowth of hair follicle-like structures were achieved
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影响因子：
3.6
作者：
Driskell RR;Jahoda CA;Chuong CM;Watt FM;Horsley V
通讯作者：
Horsley V
影响因子：
6.5
作者：
Jindo, T;Tsuboi, R;Ogawa, H
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Ogawa, H
影响因子：
3.7
作者：
Leng, LJ
通讯作者：
Leng, LJ
影响因子：
4.6
作者：
Li, Shue;Poche, John Nicholas;Hayes, Daniel J.
通讯作者：
Hayes, Daniel J.
影响因子：
3.7
作者：
Walcott BP;Kuklina EV;Nahed BV;George MG;Kahle KT;Simard JM;Asaad WF;Coumans JV
通讯作者：
Coumans JV