The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males.
The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males.
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DOI：
10.1371/journal.pgen.1010280
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发表时间：
2022-06
期刊：
影响因子：
4.5
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中科院分区：
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白纹伊蚊是世界上最具入侵性的昆虫之一，也是许多重要虫媒病毒的有效媒介。我们之前报道过Ae。白纹伊蚊（AalNix）是该物种的雄性决定因素。然而，仅AalNix是否足以启动男性发育尚不清楚。利用piggyBac转化获得了表达来自天然启动子的三种AalNix亚型的转基因系。我们验证了AalNix异构体在转基因系中的稳定表达，并证实AalNix异构体3和4足以将雌性转化为与野生型雄性无法区分的可育雄性（伪雄性）。我们还建立了一个稳定的性别转换雌蚊，AalNix3&4-♂4-伪雄。伪雄蚊可与野生型雌蚊正常交配，但其交配竞争力低于野生型雌蚊。这项工作进一步阐明了AalNix在性别决定通路中的作用，并将促进Ae的发展。白纹伊蚊控制策略依赖于雄性释放，如SIT和性别比例扭曲。在两种最重要的蚊媒病毒载体伊蚊和伊蚊中，Nix是保守的雄性决定因子。埃及伊蚊和伊蚊。蚊。仅AaeNix就可以转换Ae。埃及伊蚊雌性转化为有生育能力但不会飞的雄性。AalNix有四个可选择的剪接异构体，而AaeNix只有一个。以前很少有人知道哪个AalNix异构体是决定性别的主要信号。我们克隆了AalNix基因的启动子区域，构建了基于piggyback的不同亚型的AalNix过表达构建。在转基因株系的转化和恢复过程中，我们观察到AalNix3&4亚型的表达可以将性别决定基因的选择性剪接（双性和无果）从雌性亚型转变为雄性亚型，并在表型上使雌性雄性化或完全将雌性转化为雄性。重要的是，性别转换的伪雄性具有生育能力和飞行能力。因此，AalNix是白纹伊蚊雄性性别决定的主要信号，为性别隔离和进一步cas9介导的基因驱动种群抑制提供了基础。
Aedes albopictus is one of the most invasive insect species in the world and an effective vector for many important arboviruses. We reported previously that Ae. albopictus Nix (AalNix) is the male-determining factor of this species. However, whether AalNix alone is sufficient to initiate male development is unknown. Transgenic lines that express each of the three AalNix isoforms from the native promoter were obtained using piggyBac transformation. We verified the stable expression of AalNix isoforms in the transgenic lines and confirm that one isoform, AalNix3&4, is sufficient to convert females into fertile males (pseudo-males) that are indistinguishable from wild-type males. We also established a stable sex-converted female mosquito strain, AalNix3&4-♂4-pseudo-male. The pseudo-male mosquitoes can fly and mate normally with wild-type female, although their mating competitiveness is lower than wild-type. This work further clarifies the role of AalNix in the sex determination pathway and will facilitate the development of Ae. albopictus control strategies that rely on male-only releases such as SIT and sex-ratio distortion. Nix serves as a conserved male-determining factor in the two most important mosquito arboviral vectors, Ae. aegypti and Ae. albopictus. AaeNix alone can convert Ae. aegypti females into fertile but flightless males. AalNix has four alternative splice isoforms whereas AaeNix has one. Little was known previously about which AalNix isoform(s) serve as the primary signal for sex determination. We cloned the promoter region of AalNix gene and constructed piggybac-based AalNix overexpression constructs with different isoform variants. Following transformation and recovery of transgenic lines, we observed that expression of the AalNix3&4 isoform could shift the alternative splicing of the sex determination genes, doublesex and fruitless, from female to male isoforms, and phenotypically masculinize females or completely convert females into males. Importantly, the sex-converted pseudo-males are fertile and capable of flight. Thus, AalNix is the primary signal for male sex determination in Aedes albopictus and provides a basis for sex segregation and further Cas9-mediated gene-drive population suppression.
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DOI：
10.3390/ijerph14091006
发表时间：
2017-09-02
影响因子：
--
作者：
Macias VM;Ohm JR;Rasgon JL
通讯作者：
Rasgon JL
影响因子：
56.9
作者：
HOSHIJIMA, K;INOUE, K;SHIMURA, Y
通讯作者：
SHIMURA, Y
影响因子：
7.7
作者：
Criscione, Frank;Qi, Yumin;Tu, Zhijian
通讯作者：
Tu, Zhijian
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z
影响因子：
10.5
作者：
RYNER, LC;BAKER, BS
通讯作者：
BAKER, BS