Characterization of cell death inducing Phytophthora capsici CRN effectors suggests diverse activities in the host nucleus.
Characterization of cell death inducing Phytophthora capsici CRN effectors suggests diverse activities in the host nucleus.
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细胞死亡的表征诱导植物膜状CRN效应子表明宿主核中的活性各不相同。
DOI：
10.3389/fpls.2013.00387
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发表时间：
2013
影响因子：
5.6
通讯作者：
Huitema E
中科院分区：
文献类型：
--
作者：
Stam R;Howden AJ;Delgado-Cerezo M;M M Amaro TM;Motion GB;Pham J;Huitema E
植物 - 微生物相互作用是复杂的关联，可以通过植物免疫系统识别病原体相关的分子模式，并通过病原体编码的分泌效应子对随后的反应进行抑制。现在在一系列测序的微生物基因组中鉴定出大型效应子曲线，许多关注集中在理解其在免疫或疾病中的作用。这些研究不仅允许鉴定病原体毒力因子和策略，还提供了一个重要的分子刀具集，适用于研究植物的免疫力。细胞内效应子曲目编码大量的蛋白质，这些蛋白质会转移到宿主细胞中，并仅针对宿主核。最近的功能研究已将CRN蛋白家族作为一类重要的不同效应子，这些效应因子靶向不同的亚核室并修改宿主细胞信号传导。在这里，我们表征了三个诱导CRN的坏死，并表明细胞死亡水平存在差异。我们表明，只有CRN20_624的表达对PAMP诱导的细胞死亡具有加性作用，而AVR3A诱导的ETI则没有。鉴于它们独特的表型，我们用一组核标记评估了每个CRN的定位，并发现CRN亚核分布模式的明显差异。这些测定法还表明，CRN83_152的表达导致核染色质组织的明显变化，这表明一系列不同的事件导致过表达后细胞死亡。综上所述，我们的结果表明，CRN C-termini携带的各种功能可以被利用以识别在宿主核中发生的新过程，并且是免疫或易感性所必需的。
Plant-Microbe interactions are complex associations that feature recognition of Pathogen Associated Molecular Patterns by the plant immune system and dampening of subsequent responses by pathogen encoded secreted effectors. With large effector repertoires now identified in a range of sequenced microbial genomes, much attention centers on understanding their roles in immunity or disease. These studies not only allow identification of pathogen virulence factors and strategies, they also provide an important molecular toolset suited for studying immunity in plants. The Phytophthora intracellular effector repertoire encodes a large class of proteins that translocate into host cells and exclusively target the host nucleus. Recent functional studies have implicated the CRN protein family as an important class of diverse effectors that target distinct subnuclear compartments and modify host cell signaling. Here, we characterized three necrosis inducing CRNs and show that there are differences in the levels of cell death. We show that only expression of CRN20_624 has an additive effect on PAMP induced cell death but not AVR3a induced ETI. Given their distinctive phenotypes, we assessed localization of each CRN with a set of nuclear markers and found clear differences in CRN subnuclear distribution patterns. These assays also revealed that expression of CRN83_152 leads to a distinct change in nuclear chromatin organization, suggesting a distinct series of events that leads to cell death upon over-expression. Taken together, our results suggest diverse functions carried by CRN C-termini, which can be exploited to identify novel processes that take place in the host nucleus and are required for immunity or susceptibility.
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phytophthora capsici-tomato相互作用具有与半生物营养性生活方式相关的基因表达的急剧变化。
影响因子：
12.3
作者：
Jupe J;Stam R;Howden AJ;Morris JA;Zhang R;Hedley PE;Huitema E
通讯作者：
Huitema E
影响因子：
12.3
作者：
Lévesque CA;Brouwer H;Cano L;Hamilton JP;Holt C;Huitema E;Raffaele S;Robideau GP;Thines M;Win J;Zerillo MM;Beakes GW;Boore JL;Busam D;Dumas B;Ferriera S;Fuerstenberg SI;Gachon CM;Gaulin E;Govers F;Grenville-Briggs L;Horner N;Hostetler J;Jiang RH;Johnson J;Krajaejun T;Lin H;Meijer HJ;Moore B;Morris P;Phuntmart V;Puiu D;Shetty J;Stajich JE;Tripathy S;Wawra S;van West P;Whitty BR;Coutinho PM;Henrissat B;Martin F;Thomas PD;Tyler BM;De Vries RP;Kamoun S;Yandell M;Tisserat N;Buell CR
通讯作者：
Buell CR
影响因子：
11.6
作者：
Dou, Daolong;Kale, Shiv D.;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
3.7
作者：
Gaulin E;Madoui MA;Bottin A;Jacquet C;Mathé C;Couloux A;Wincker P;Dumas B
通讯作者：
Dumas B
影响因子：
3.5
作者：
Bos, Jorunn I. B.;Chaparro-Garcia, Angela;Kamoun, Sophien
通讯作者：
Kamoun, Sophien