Anti-diabetic Effects of Clostridium butyricum CGMCC0313.1 through Promoting the Growth of Gut Butyrate-producing Bacteria in Type 2 Diabetic Mice.
Anti-diabetic Effects of Clostridium butyricum CGMCC0313.1 through Promoting the Growth of Gut Butyrate-producing Bacteria in Type 2 Diabetic Mice.
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丁酸梭菌 CGMCC0313.1 通过促进 2 型糖尿病小鼠肠道产丁酸细菌生长的抗糖尿病作用
DOI：
10.1038/s41598-017-07335-0
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发表时间：
2017-08-01
影响因子：
4.6
通讯作者：
Chen YQ
中科院分区：
文献类型：
--
作者：
Jia L;Li D;Feng N;Shamoon M;Sun Z;Ding L;Zhang H;Chen W;Sun J;Chen YQ
2型糖尿病(T2D)患者的丁酸产生菌减少。我们推测，补充丁酸产生菌可能会对T2D产生有益影响。本研究调查了具有良好特性的丁酸产生菌酪酸梭菌CGMCC0313.1(CB0313.1)对两种糖尿病小鼠模型的高血糖和相关代谢功能障碍的影响。CB0313.1从3周龄开始连续灌胃给Leptindb/db小鼠5周，对C57BL/6J小鼠高脂饮食(HFD)加链脲佐菌素(STZ)诱导的HF糖尿病小鼠从4周龄开始连续13周灌胃。CB0313.1可改善糖尿病标志物(空腹血糖、糖耐量、胰岛素耐量、GLP-1和胰岛素分泌)，降低血脂和炎症张力。此外，CB0313.1还能逆转肝功能低下，减少葡萄糖输出。我们还发现，CB0313.1调节了肠道微生物区系组成，其特征是菲米米特和类杆菌的比率降低，减少了HF糖尿病小鼠中丰富的Allobaculum细菌，并增加了丁酸盐的产生细菌。CB0313.1治疗后肠道微生物区系的变化与过氧化物酶体增殖物激活受体γ(PPARγ)、胰岛素信号分子和线粒体功能标志物的增强有关。总之，我们的研究表明，CB0313.1可能是一种有益的益生菌，用于预防和治疗高血糖和相关的代谢功能障碍。
Patients with type 2 diabetes (T2D) have decreased butyrate-producing bacteria. We hypothesized that supplementation with butyrate-producing bacteria may exert beneficial effects on T2D. The current study investigated the effects of well-characterized butyrate-producing bacteria Clostridium butyricum CGMCC0313.1 (CB0313.1) on hyperglycemia and associated metabolic dysfunction in two diabetic mouse models. CB0313.1 was administered daily by oral gavage to leptindb/db mice for 5 weeks starting from 3 weeks of age, and to HF diabetic mice induced by high fat diet (HFD) plus streptozotocin (STZ) in C57BL/6J mice for 13 weeks starting from 4 weeks of age. CB0313.1 improved diabetic markers (fasting glucose, glucose tolerance, insulin tolerance, GLP-1 and insulin secretion), and decreased blood lipids and inflammatory tone. Furthermore, CB0313.1 reversed hypohepatias and reduced glucose output. We also found that CB0313.1 modulated gut microbiota composition, characterized by a decreased ratio of Firmicutes to Bacteroidetes, reduced Allobaculum bacteria that were abundant in HF diabetic mice and increased butyrate-producing bacteria. Changes in gut microbiota following CB0313.1 treatment were associated with enhanced peroxisome proliferator–activated receptor-γ (PPARγ), insulin signaling molecules and mitochondrial function markers. Together, our study suggests that CB0313.1 may act as a beneficial probiotic for the prevention and treatment of hyperglycemia and associated metabolic dysfunction.
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影响因子：
2.7
作者：
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影响因子：
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作者：
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