Multi-channel PMMA microfluidic biosensor with integrated IDUAs for electrochemical detection.
Multi-channel PMMA microfluidic biosensor with integrated IDUAs for electrochemical detection.
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DOI：
10.1007/s00216-013-7020-0
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发表时间：
2013-07
影响因子：
4.3
通讯作者：
Baeumner AJ
中科院分区：
文献类型：
--
作者：
Wongkaew N;He P;Kurth V;Surareungchai W;Baeumner AJ
研制了一种新型的多通道聚甲基丙烯酸甲酯（PMMA）微流控生物传感器，该传感器具有交叉式超微电极阵列（IDUAs），用于电化学检测。开发的重点是一个简单的制造过程和实现一个可靠的大型IDUA，可以同时提供多个微通道的检测。作为概念验证，五个微通道被放置在一个大的单个IDUA上，其中通道与电极指的长度平行。在PMMA覆盖片上制备了IDUAs，并使用UV/臭氧辅助热键连接到含有微流控通道的PMMA衬底上。优化了器件制造条件，实现了粘合PMMA器件内的坚固型大型IDUA。优化了金与PMMA的附着力、保护涂层和键合压力。采用安培法检测铁酸钾和六氰化铁，研究了其电化学性能。同一芯片内的累积信号在0 ~ 38 μM范围内具有良好的线性（R2 = 0.98），检测限为3.48 μM。该装置的键合进行了优化，因此没有观察到通道之间的串扰，否则将导致不可靠的电化学响应。所获得的高度可再现的信号与使用单独的单通道设备获得的信号相当。随后，将多通道微流控芯片应用于模型生物分析检测策略，即使用三明治方法对特定核酸序列进行定量。在这里，用探针包被的顺磁珠和探针标记的脂质体包裹铁/六氧化氰化铁作为氧化还原标记物，与单链DNA序列结合。优化了用于脂质体裂解的非离子型洗涤剂正辛基-β- d -葡萄糖吡喃苷（OG）的流速，在250 s内库伦法检测目标序列，检测限为12.5 μM。与先前发表的单通道设计相比，设计的稳健性和结果的可靠性表明，多通道设备为需要多分析物检测和高通量分析的生物分析应用提供了极好的机会。
A novel multi-channel poly(methyl methacrylate) (PMMA) microfluidic biosensor with interdigitated ultramicroelectrode arrays (IDUAs) for electrochemical detection was developed. The focus of the development was a simple fabrication procedure and the realization of a reliable large IDUA that can provide detection simultaneously to several microchannels. As proof of concept, five microchannels are positioned over a large single IDUA where the channels are parallel with the length of electrode finger. The IDUAs were fabricated on the PMMA cover piece and bonded to a PMMA substrate containing the microfluidic channels using UV/ozone-assisted thermal bonding. Conditions of device fabrication were optimized realizing a rugged large IDUA within a bonded PMMA device. Gold adhesion to the PMMA, protective coatings and pressure during bonding were optimized. Its electrochemical performance was studied using amperometric detection of potassium ferri and ferro hexacyanide. Cumulative signals within the same chip showed very good linearity over a range of 0 - 38 μM (R2 = 0.98) and a limit of detection of 3.48 μM. The bonding of the device was optimized so that no cross-talk between the channels was observed which otherwise would have resulted in unreliable electrochemical responses. The highly reproducible signals achieved were comparable to those obtained with separate single-channel devices. Subsequently, the multi-channel microfluidic chip was applied to a model bioanalytical detection strategy, i.e. the quantification of specific nucleic acid sequences using a sandwich approach. Here probe-coated paramagnetic beads and probe-tagged liposomes entrapping ferri/ferro hexacyanide as the redox marker were used to bind to a single stranded DNA sequence. Flow rates of the non-ionic detergent n-octyl-β-D-glucopyranoside (OG) for liposome lysis were optimized and the detection of the target sequences was carried out coulometrically within 250 s and with a limit of detection of 12.5 μM. The robustness of the design and the reliability of the results obtained in comparison to previously published single-channel designs suggest that the multi-channel device offers an excellent opportunity for bioanalytical applications that require multi-analyte detection and high throughput assays.
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DOI：
10.1007/s00542-008-0694-0
发表时间：
2009-03-01
影响因子：
2.1
作者：
Nugen, S. R.;Asiello, P. J.;Baeumner, A. J.
通讯作者：
Baeumner, A. J.
集成多室聚合酶链式反应和多通道分离的遗传分析微流控装置
影响因子：
6.2
作者：
Pan, Xiaoyan;Jiang, Lei;Qin, Jianhua
通讯作者：
Qin, Jianhua
影响因子：
13.1
作者：
Hervas, Mirian;Lopez, Miguel A.;Escarpa, Alberto
通讯作者：
Escarpa, Alberto
影响因子：
7.4
作者：
Amatore, Christian;Da Mota, Nicolas;Thouin, Laurent
通讯作者：
Thouin, Laurent
影响因子：
6.1
作者：
Goral, VN;Zaytseva, NV;Baeumner, AJ
通讯作者：
Baeumner, AJ